Anti-Lamin A (mature) Antibody, clone 4A4-A4

32160702

NA.41

Nuclear lamins are composed of the type V intermediate filament proteins. Often referred to as nucleoskeletal proteins they play a key role in nuclear integrity, positioning of nuclear pores, and overall nuclear size and shape. They also play several key functional roles in the maintenance and propagation of the genome--replication, transcription-- as well as the disassembly and reassembly of the nucleus during cell division. In vitro studies have shown that dimers are the basic building blocks of higher order lamin structures and in low concentrations lamins are distributed throughout the nucleoplasm. In humans, there are two types of lamins: A-type lamins (lamins A and C), found primarily in differentiated cells, and B-type lamins (lamins B1 and B2), found in all nucleated cells. Nuclear lamins are involved in a number of essential nuclear functions, including nuclear envelope assembly and disassembly during cell division, DNA synthesis, transcription, and apoptosis. Nuclear lamins have been found to co-localize with DNA synthesis sites.

KLH-conjugated Peptide corresponding to the C-terminus of human Lamin A (mature).
Epitope: C-terminus

Research Category
Cell Structure
Immunoprecipitation Analysis: A representative lot immunoprecipitated Lamin A (mature) in wild type mouse fibroblasts cell lysate.
Immunocytochemistry Analysis: A representative lot detected Lamin A (mature) in wild type mouse fibroblasts cells.
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Lamin A (mature) in 10 µg of A431 and THP-1 cell lysate.
Western Blotting Analysis: A representative lot detected Lamin A (mature) in EDMD and FPLD fibroblasts (Roblek, M., et al. (2010). PLOS ONE. 5(5):e10604).
Western Blotting Analysis: A 1:100 dilution from a representative lot detected Lamin A (mature) in pre-lamin A and Lamin A (mature).
Anti-Lamin A (mature), clone 4A4-A4 is an antibody against Lamin A (mature) for use in WB, IP, ICC.
Research Sub Category
Adhesion (CAMs)

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Lamin A (mature) in 10 µg of Jurkat cell lysate.

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

Concentration: Please refer to lot specific datasheet.

100

mouse

purified immunoglobulin

primary antibodies

4A4-A4, monoclonal

human

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

IgG1κ

NP_001244303

P02545

wet ice

human ... LMNA(4000)

~70 kDa observed

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1