Anti-Isocitrate Dehydrogenase 1 (IDH1)-R132H Antibody, clone HMab-1

32160702

NA.41

Isocitrate dehydrogenase 1 (IDH1) is a 46 kDa NADP-dependent enzyme, which catalyzes the decarboxylation of isocitrate into α-ketoglutarate. IDH1 may also play a role in the prevention of oxidative damage, and the shuttling of proteins to peroxisomes. It is widely reported that mutations in IDH1 result in various types of glioma.

This antibody is specific for the R132H mutation of Isocitrate Dehydrogenase 1 (IDH1).

KLH-conjugated linear peptide corresponding to human Isocitrate Dehydrogenase 1 (IDH1).

Western Blot Analysis: A representative lot of this antibody detected Isocitrate Dehydrogenase 1 (IDH1)-R132S in a panel of wild-type and mutated recombinant IDH1 proteins.

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Isocitrate Dehydrogenase 1 (IDH1)-R132S in anaplastic and diffuse astrocytoma tissues and in primary glioblastoma tissues (Kato, Y., et al. (2009). Biochem Biophys Res Commun. 390(3):547-51.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Isocitrate Dehydrogenase 1 (IDH1)-R132S in transfected glioblastoma and transfected LN229 cells (Kaneko, M. K., et al. (2011). Biochem Biophys Res Commun. 406(4):608-613.).
Research Sub Category
Apoptosis - Additional
Detect Isocitrate Dehydrogenase 1 (IDH1)-R132H using this Anti-Isocitrate Dehydrogenase 1 (IDH1)-R132H Antibody, clone HMab-1 validated for use in Western Blotting, IHC, ICC.
Research Category
Apoptosis & Cancer

Evaluated by Western Blot in MBP-tagged recombinant Isocitrate Dehydrogenase 1 (IDH1).

Western Blot Analysis: A 1:500 dilution of this antibody detected MBP tagged recombinant Isocitrate Dehydrogenase 1 (IDH1) in WB.

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

Control
MBP tagged recombinant Isocitrate Dehydrogenase 1 (IDH1)

100

mouse

purified immunoglobulin

primary antibodies

HMab-1, monoclonal

human

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

IgG1κ

NP_005887

O75874

wet ice

human ... IDH1(3417)

~90 kDa observed. The calculated molecular weight of Isocitrate Dehydrogenase 1 (IDH1) is 47 kDa However, a Western Blot was performed using an MBP-tagged recombinant Isocitrate Dehydrogenase 1 (IDH1). Because MBP has a molecular weight of ~42 kDa, a band was observed at ~90 kDa

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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WGK 1