Anti-Human B cells (CD19) Antibody, clone FMC63

32160702

BIOCHEMISTRY:

p I = 7.0

This specificity of this antibody is equivalent to that of CD19 monoclonal antibodies (Zola et al., 1988, 1989), based on comparative studies, antigen recognition and positive reaction with a CD19 transfected cell line. It detects a 96 kD single chain glycoprotein on the surface of human B lineage cells, including pre-B cells. B cells can be detected in peripheral blood and tissues. The antibody reacts with most B cell malignancies including chronic lymphocytic leukemia and most lymphomas, but not multiple myeloma.

Cell reactivity:

Normal:Stains all B lymphocytes in peripheral blood and spleen. Negative on granulocytes, monocytes, platelets, erythrocytes and T lymphocytes.

Clinical:

B cell chronic lymphocytic leukemia (B-CLL) +

Pro lymphocytic leukemia (PLL) +

Hairy cell leukemia (HCL) +

Common acute lymphoblastic leukemia (CALL) +

Pre-B acute lymphoblastic leukemia (pre-B-ALL) +

NULL-acute lymphoblastic leukemia (NULL-ALL) +

T common lymphocytic leukemia (T-CLL) -

Raji cells

This Anti-Human B cells CD19 Antibody, clone FMC63 is validated for use in FC, IF for the detection of CD19.
This antibody is useful for enumerating normal B lymphocytes and typing B cell malignancies by flow cytometry and immunofluorescence.

SUGGESTED USAGE DILUTION

Flow cytometry and Indirect Immunofluorescence 1:25

Dilute with isotonic buffer. Use 50 μl of diluted antibody per 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.
Research Sub Category
Immunoglobulins & Immunology
Research Category
Inflammation & Immunology

Replaces: CBL141

Purified from mouse ascitic fluid. The antibody is supplied in 25 mM Tris-HCl buffer, 0.4 M NaCl, pH 8.0, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis and flow cytometry.
Format: Purified

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

purified antibody

primary antibodies

FMC63, monoclonal

human

Chemicon^®

flow cytometry: suitable
immunofluorescence: suitable

IgG2a

NM_001770.4

P15391

wet ice

human ... CD19(930)

Store at 2 to 8°C, for up to 6 months. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES.

WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2