Anti-GPR56, C-terminal Antibody

32160702

NA.41

G-protein coupled receptor 56 (GPR56) is a ubiquitous adhesion receptor, which belongs to the G-protein coupled receptor 2 family and LN-TM7 subfamily. It is abundantly expressed in the in thyroid gland, brain, heart, and various tumor cells. Previous studies have suggested that GPR56 may inhibit the progression and metastasis of melanomas by interaction with extracellular-matrix proteins, such as Tissue Transglutaminase. GPR56 also plays a role in regulating VEGF production and angiogenesis via a PKCα-mediated pathway. GPR56 also plays a critical role in the development of the frontal cortex; previous studies have reported that mutations in GPR56 result in disorganized cortical lamination, which is most pronounced in the frontal cortex. This condition is known as bilateral frontoparietal polymicrogyria or BFPP.

linear peptide corresponding to the C-terminus of Mouse GPR56.

Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipated GPR56 from rat kidney cell lysate and visualized through western blot (Paavola, K., et al. (2011). J. Biol. Chem. 2011, 286:28914-28921.)
Western Blot Analysis: A representative lot of this antibody detected GPR56 from rat kidney cell lysate (Paavola, K., et al. (2011). J. Biol. Chem. 2011, 286:28914-28921.)
Immunocytochemistry Analysis: A representative lot of this antibody detected GPR56 from transfected HEK293 cells (Paavola, K., et al. (2011). J. Biol. Chem. 2011, 286:28914-28921.)
This Anti-GPR56, C-terminal Antibody is validated for use in Western Blotting and Immunoprecipitation and Immunocytochemistry for the detection of GPR56, C-terminal.

Evaluated by Western Blotting in Hela cell lysate.

Western Blotting Analysis: 1 µg/ml of this antibody detected GPR56 in 10 µg of Hela cell lysate.

Antigen Affinity Purified

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

human, rat

mouse (based on 100% sequence homology)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NP_001185823

Q8K209

wet ice

human ... GPR56(9289)

~ 120 kDa observed
This receptor is heavily glycosylated in cells, and thus the apparent molecular mass of the full-length receptor in most cell types is around 90-120 kDa Moreover, this receptor is prone to extensive post-translational processing and proteolysis, and thus depending on the cell type the most prominent immunoreactive bands on Western blot are usually around 45 kDa, 25 kDa and/or 15 kDa in size

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12 - Non Combustible Liquids

WGK 1