Anti-Dopamine D2 Receptor Antibody

32160702

NA.41

Recognizes Dopamine D2 Receptor (D2R). Recognizes both native and denatured forms of D2R (110, 68 and 47 kDa bands by Western blot using rat striatal positive control (Catalog Number AG282)). AB1558 inhibits binding of [3H]YM-09152-2, a known D2R antagonist and (125)I-NAPS, a photoligand. It inhibits binding of [3H]YM-09152-2 and PPHT-fluorescein (an agonist of D2R) and reacts with 110 kDa D2R band in fibroblasts transfected with rat D2R cDNA. AG284 is available for blocking experiments; AG284 is not recommended as a positive control for western blots.

Amino-terminal peptide sequence (aa 24-34) near or at the ligand binding domain of rat D2R (1,2).

The immunogen shows 100% homology with mouse, 90% with human and bovine and 72% with canine D2R receptors. An antibody made to the same sequence has shown reactivity with human, lobster, squid and crawfish.

Immunohistochemistry: 4% PFA fixed, frozen sections, 1:200, 24 hour incubation at room temperature, DAB detection. {Tillerson, JL et al. (2006) Behavioural Brain Res. 172:97-105.

Immunocytochemistry: 4% PFA fixed cells 10-15′, permeabilized with 0.2-0.3% triton X-100, 1:100, o/N 4C for fluorescence {Sun, W et al (2005) Stem Cells 23(7):931-945, http://stemcells.alphamedpress.org/cgi/content/full/23/7/931}.

Western blot: 1:1,000-1:5,000 (Chemiluminescence technique) Higher concentration of antibody may be necessary when using low concentration samples or when using milk in antibody diluents. It may be necessary to load 100-200 μg of purified striatal or other tissue membranes to visualize the DR2 bands. The antibody has been shown to recognize both native and denatured DR2 bands.

ELISA: 1:10,000-1:50,000 [1 μg/mL D2R peptide (Cat. Number AG284)/well]

Optimal working dilutions must be determined by end user.
Anti-Dopamine D2 Receptor Antibody is an antibody against Dopamine D2 Receptor for use in ELISA, IC, IH & WB.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Unpurified
Rabbit antiSerum. Liquid containing 0.05% sodium azide.

Control
Brain

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

serum

primary antibodies

polyclonal

rat

Chemicon^®

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

NM_000795.2
NM_016574.2

P14416

dry ice

110, 68 and 47 kDa

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 1