Anti-DNA polymerase delta p125 Antibody, clone 11E10.1

32160702

NA.41

DNA polymerase delta catalytic subunit (EC 2.7.7.7; UniProt P28340; also known as CDC2 homolog, DNA polymerase subunit delta p125) is encoded by the POLD1 (also known as CDC2, MDPL, POLD, CRCS10) gene (Gene ID 5424) in human. DNA replication in eukaryotes involves multiple enzymes including the B family polymerases Pol α, Pol δ, and Pol ɛ. While Pol α initiates DNA synthesis, Pol δ and Pol ɛ replace Pol α after primer extension and perform the bulk of DNA replication. Pol δ and Pol ɛ are heterotetramers in higher eukaryotes comprised of POLD1 and POLE as the respective catalytic subunit, as well as accessory subunits (POLD2/3/4 and POLE2/3/4) that interact with cofactors such as Proliferating Cell Nuclear Antigen (PCNA). Both POLD1 and POLE contain an exonuclease domain (a.a. 268–471 of POLE and 304–517 of POLD1) that proof-reading capability during DNA replication. In addition to DNA synthesis, Pol ɛ and Pol δ are believed to participate in chromosomal DNA repairs, including nucleotide excision repair (NER), ismatch repair (MMR) and repair of double strand breaks (DSBR). Germline exonuclease domain mutations (EDMs) in POLD1 and POLE cause polymerase proofreading-associated polyposis (PPAP). Tumors with EDMs exhibits dramatically increased base substitutions.

GST-tagged recombinant protein corresponding to the N-terminus of human DNA polymerase delta p125.

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected DNA polymerase delta p125 in 10 µg of Ramos cell lysate.
Anti-DNA polymerase delta p125 Antibody, clone 11E10.1 is an antibody against DNA polymerase delta p125 Antibody for use in Western Blotting.

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected DNA polymerase delta p125 in 10 µg of Raji cell lysate.

Protein G Purified
Format: Purified

Concentration: Please refer to lot specific datasheet.

100

mouse

purified immunoglobulin

primary antibodies

11E10.1, monoclonal

human

western blot: suitable

IgG2bκ

NP_001243778

P28340

wet ice

human ... POLD1(5424)

~125 kDa observed.

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WGK 1