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Anti-DNA helicase B (HELB)

产品编号:4107101
规格:from rabbit
包装规格:25 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
410710125 μG1420
产品别名

Anti-DNA helicase B (HELB)

DNA helicase B, hDHB

基本信息
eCl@ss
32160702
General description【一般描述】
DNA helicase B (UniProt: Q8NG08; also known as EC: 3.6.4.12, hDHB) is encoded by the HELB gene (Gene ID: 92797) in human. DNA helicase B is highly expressed in testis and thymus and weaker expression is observed in liver, spleen, kidney, and brain. It is predominantly nuclear, however, following phosphorylation at serine 967 by CDK2 during the G1/S transition it is exported into the cytoplasm. Following DNA damage, it is recruited to sites of double-strand breaks by the RPA complex. Its recruitment to single-stranded DNA following DNA damage leads to inhibition of nucleases catalyzing resection, such as EXO1, BLM and DNA2, possibly via the 5′-3′ ssDNA translocase activity of HELB. As cells approach S phase, DNA end resection is promoted by the nuclear export of HELB following its phosphorylation. Its nuclear export signal is localized to amino acids 1055-1078. It uses either magnesium or manganese ions to support its helicase activity and the DNA helicase B activity is inhibited by salt concentrations higher than 100 mM. It binds strongly to single-stranded DNA in the absence of ATP, but dissociates readily in the presence of 1 mM ATP. Two isoforms of DNA helicase B have been reported that are produced by alternative splicing.
Specificity【特异性】
This rabbit polyclonal antibody specifically detects DNA helicase B in human cells.
Immunogen【免疫原】
MBP-conjugated recombinant fragment corresponding to the first 199 amino acids from the N-terminal region of human DNA helicase B.

Epitope: N-terminus
Application【应用】
Research Category
Epigenetics & Nuclear Function
Western Blotting Analysis: A representative lot detected DNA helicase B (HELB) in U2OS cells transfected with a non-targeting siRNA (CTRL) or siRNAs targeting protein and HeLa cells transfected with non-targeting (siCTRL) or HELB-targeting (siHELB) siRNAs (Tkac, J., et. al. (2016). Mol Cell. 61(3):405-418).

Anti-DNA helicase B (HELB) Antibody, Cat. No. ABE2601, is a highly specific rabbit polyclonal antibody that targets DNA helicase B (HELB) and has been tested for use in Western Blotting.
Quality【质量】
Evaluated by Western Blotting in siRNA control & siRNA HELB U2OS cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected DNA helicase B (HELB) in lysate from U2OS cells treated with non-targeting siRNA, but not in lysates from siRNA HELB treated U2OS cells.


Physical form【外形】
Format: Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified
Other Notes【其他说明】
Concentration: Please refer to lot specific datasheet.
产品性质
biological source【生物来源】
rabbit
antibody form【抗体形式】
affinity isolated antibody
antibody product type
primary antibodies
clone【克隆】
polyclonal
species reactivity
human
packaging【包装】
antibody small pack of 25 μg
technique(s)
western blot: suitable
isotype【同位素/亚型】
IgG
NCBI accession no.【NCBI登记号】
NP_387467
UniProt accession no.【UniProt登记号】
Q8NG08
产品说明
Target description【目标描述】
~145 kDa observed; 123.25 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Storage and Stability【储存及稳定性】
Stable for 1 year at 2-8°C from date of receipt.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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