Anti-Collagen Type IV α 2 Chain Antibody, clone 23IIC3

32160702

NA.41

Recognizes Human type IV collagen, alpha-2 chains.

Human amniotic type IV collagen

Research Category
Cell Structure
Detect Collagen Type IV α 2 Chain using this Anti-Collagen Type IV α 2 Chain Antibody, clone 23IIC3 validated for use in ELISA, IH(P) & WB.
Immunohistochemistry: 1:500, acetone fixed, frozen tissue recommended. Weak reactivity on on formalin fixed, paraffin sections (1:100) after treatment with 1% trypsin (20 minutes, room temperature). Or alternatively, 4% PFA fixed, HEIR treated tissue has worked in monkey (Qin, 2003) at 1:200 dilution.

Western blot: 1:500, 170 kDa.

ELISA at 1:200

Optimal working dilutions must be determined by the end user.
Research Sub Category
ECM Proteins

Unpurified
Liquid.

Control
Positive Control: Kidney, muscle, tendon spleen tissue
Negative Control: Neurons/glia

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

ascites fluid

primary antibodies

23IIC3, monoclonal

monkey, human, rat

Chemicon^®

ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

IgG1

P08572

dry ice

human ... COL4A2(1284)

170 kDa

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 1