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Anti-Cohesin subunit SA-2 Antibody
| 产品编号： | 4106406 |
| 规格： | from rabbit, purified by affinity chromatography |
| 包装规格： | 100 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-Cohesin subunit SA-2 Antibody
Cohesin subunit SA-2, SCC3 homolog 2, Stromal antigen 2
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | The cohesin subunit SA-2 is a part of the cohesin complex. This complex is comprised of a heterodimer between SMC1 and SMC3 proteins, as well as RAD21 and STAG proteins (STAG1, 2, or 3). These proteins form a large ring that can trap sister chromatids after DNA replication. During prophase, the cohesion complexes dissociate from the chromatin due to the phosphorylation by PLK1 (Polo-like kinase) with the exception at the centromeres where the cohesion complexes remain. Then during anaphase, the RAD21 subunit is cleaved which causes the dissociation of the complex from chromosomes, allowing chromosome separation. |
| Specificity【特异性】 | Other homologies: Mouse and Rat (93%-14/15aa). |
| Immunogen【免疫原】 | KLH-conjugated linear peptide corresponding to human Cohesin Subunit SA-2. |
| Application【应用】 | Research Category Epigenetics & Nuclear Function Research Sub Category Chromatin Biology Detect Cohesin subunit SA-2 using this Anti-Cohesin subunit SA-2 Antibody validated for use in 0. Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Cohesin Subunit SA-2 in A431 and HeLa cells. Immunoprecipitation Analysis: A representative lot immunoprecipitated Cohesin Subunit SA-2 from HeLa cell lysate. |
| Quality【质量】 | Evaluated by Western Blot in HeLa cell nuclear extract. Western Blot Analysis: 0.2 µg/mL of this antibody detected Cohesin Subunit SA-2 in 10 µg of HeLa cell nuclear extract. |
| Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Affinity purified |
| Analysis Note【分析说明】 | Control HeLa cell nuclear extract |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | human |
| species reactivity (predicted by homology) | rhesus monkey (based on 100% sequence homology), equine (based on 100% sequence homology), bovine (based on 100% sequence homology) |
| technique(s) | immunocytochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NP_006594 |
| UniProt accession no.【UniProt登记号】 | Q8N3U4 |
| shipped in【运输】 | wet ice |
| Gene Information | human ... STAG2(10735) |
产品说明
| Target description【目标描述】 | ~140 kDa observed. An uncharacterized band may appear at ~35 kDa in some lysates. Uncharacterized bands may appear at ~40 kDa and ~30 kDa in Immunoprecipitation. |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
