Anti-Chondroitin 6 Sulfate Antibody, clone MK-302

32160702

NA.41

Reacts with a wide range of cartilage proteoglycans after either chondroitinase (ABC or AC) or testicular hyaluronidase digestion. Native proteoglycans do not react with MAB2035. Antibody binding to epitope is successfully inhibited by disaccharides of chondroitin-6-sulfate. Recognizes the chondroitin-6-sulfate stubs (preferentially in clusters) of high density cartilage proteoglycans of different species. No cross-reactivity with dermatan sulfate, keratan sulfate or chondroitin-4-sulfate.

Chondroitinase ABC-digested adult human aggrecan.

ELISA at 1:100-1:5,000

RIA at 1:100-1:200.

Chondroitinase ABC digestion prior to antibody reaction is required for antibody reactivity. {0.5U/mL in 100mM Tris-HCL, 30 minutes room temperature} A dramatic proteolytic digestion of the core protein (e.g. with papain or pronase) significantly reduces the antibody reactivity.

Optimal working dilutions must be determined by the end user.
Anti-Chondroitin 6 Sulfate Antibody, clone MK-302 is an antibody against Chondroitin 6 Sulfate for use in ELISA, RIA & WB.

Ascites liquid; no preservative.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

ascites fluid

primary antibodies

MK-302, monoclonal

human, mouse, sheep, bovine, canine, rat, rabbit

Chemicon^®

ELISA: suitable
radioimmunoassay: suitable
western blot: suitable

IgG1

NM_001135.2
NM_013227.2

P16112

dry ice

human ... ACAN(176)

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10 - Combustible liquids

WGK 1