Anti-Cholesterol acyltransferase 1 Antibody

32160702

NA.41

Cholesterol acyltransferase 1 (ACAT1) is mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA and plays a significant role in ketone body metabolism. Defects in the gene encoding ACAT1 are associated with the alpha-methylacetoaceticaciduria disorder,an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. Recent studies suggests that ACAT1 may be a therapeutic target for treating certain forms of Alzeheimer′s disease.

Other homologies: Mouse (93% sequence homology).

KLH-conjugated linear peptide corresponding to human Cholesterol acyltransferase 1.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Cholesterol acyltransferase 1 in human adrenal gland and normal human brain tissues.
This Anti-Cholesterol acyltransferase 1 Antibody is validated for use in WB, IH(P) for the detection of Cholesterol acyltransferase 1.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases

Evaluated by Western Blot in Caco-2 cell lysate.

Western Blot Analysis: 0.05 µg/mL of this antibody detected Cholesterol acyltransferase 1 on 10 µg of Caco-2 cell lysate.

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

Control
Caco-2 cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

human, rhesus macaque

chimpanzee (based on 100% sequence homology), rhesus monkey (based on 100% sequence homology), rat (based on 100% sequence homology)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NP_003092

P35610

wet ice

human ... SOAT1(6646)

~58 kDa observed which may be the result of dimerization/aggregation of the native protein during sample preparation for SDS-PAGE. The calculated molecular weight is 65 kDa

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1