Anti-Calpain Antibody, small subunit of μ- or m-Calpains (Calpain I or II), clone P1

32160702

NA.41

Calpains are non-lysosomal cysteine proteeases which absolutely require calcium for activity. There are two major calpain isozymes: μ-calpain and m-calpain, which require micromolar or millimolar calicum for activity, respectively, in invitro assays. The major calpain isozymes are composed of two subunits: an 80kDa catalytic subunit; and a 30kDa regulatory subunit. The large and small subunits remain associated in the presence of calcium after proteolytic activation of the zymogen.

Reacts with the small subunit of human erthrocyte μ-calpain (Calpain I) or bovine myocardial m-calpain (Calpain II).

Bovine myocardial m-calpain.
Epitope: small subunit of μ- or m-Calpains (Calpain I or II)

This Anti-Calpain Antibody, small subunit of μ-or m-Calpains (Calpain I or II), clone P1 is validated for use in ELISA, WB, IC, IH for the detection of Calpain.
Immunoblot: 1:500-1,000. The small subunit of either μ-calpain m-calpain is 30kDa. ELISA: 1:50,000 (against the immunogen). Immunohistochemistry: 1:100 on 4% paraformaldehyde or 1% paraformaldehyde/4% glutaraldehyde fixed tissue. Immunocytochemistry: 1:100. Optimal working dilutions must be determined by the end user.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

ascites fluid

primary antibodies

P1, monoclonal

human, bovine

rat

Chemicon^®

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

IgG1

NM_001003962.1
NM_001749.2

P04632

dry ice

human ... CAPNS1(826)

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10 - Combustible liquids

WGK 1