Anti-Autotaxin Antibody

32160702

NA.41

Autotaxin (also known as Enpp2), an exo-enzyme that is upregulated in various cancers such as breast and lung cancer, is a secreted protein integral to the production of the lipid signaling molecule, lysophosphatidic acid (LPA). Wutotaxin is synthesized as a pre-pro-enzyme and stimulates cell proliferation, cell motility, and angiogenesis. Studies have shown increased expression of autotaxin in tumors is associated with increased tumor aggressiveness.

This antibody recognizes Autotaxin at the substrate binding region.

Epitope: Substrate binding region
KLH-conjugated linear peptide corresponding to human Autotaxin at the substrate binding region.

Western Blot Analysis: 0.5 µg/mL from a representative lot detected Autotaxin in 10 µg of oligodendrocyte precursor cell lysate. Western Blot performed with Tween.

Immunohistochemistry Analysis: A 1:400 dilution from a representative lot detected Autotaxin in human cerebral cortex and ductal carcinoma tissues.
Detect Autotaxin using this Anti-Autotaxin Antibody validated for use in WB, IH(P).

Evaluated by Western Blot in oligodendrocyte precursor cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Autotaxin in 10 µg of oligodendrocyte precursor cell lysate. Western Blot performed without Tween.

Affinity purified

Control
Oligodendrocyte precursor cell lysate

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

human

bovine (based on 100% sequence homology), mouse (based on 100% sequence homology), rat (based on 100% sequence homology), canine (based on 100% sequence homology)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NP_001035181

Q13822

wet ice

human ... ENPP2(5168)

~100/125 kDa observed.
3 isoforms are produced by alternative splicing with molecular weights of 99, 105, and 101 kDa The calculated molecular weight of this protein is 99 kDa, but can be observed at ~125 kDa due to high glycosylation. An uncharacterized band may appear at ~35 kDa in some lysates.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1