Anti-ASF-1 Antibody, clone AK-96

32160702

NA.41

Alternative-splicing factor 1 (ASF-1), also known as SRp30a or SF2, is a member of the highly conserved family of SR proteins, a group of serine/arginine-rich domain-containing proteins that play a critical role in regulated and constitutive splicing of precursor mRNA. The SR family of proteins is characterized as having one to two RNA recognition motifs located in the n-terminal sequence, and an RS domain within the C-terminus that is rich in serine/arginine dipeptide repeats. ASF-1 has an inhibitory role in exon skipping, in order to maintain splicing accuracy and to monitor alternative splicing. Studies have shown that ASF-1 plays a role in the regulation of caspase-9 alternative splicing as well as being a critical component for ceramide to function as a mediator of alternative splicing for the same caspase.

Recombinant protein corresponding to human ASF-1.

Research Sub Category
Nuclear Receptors
Western Blotting Analysis: 1.0 µg/mL of this antibody detected ASF-1 in 10 µg of Raji and HepG2 cell lysate.
Immunocytochemistry Analysis: A representative lot detected ASF-1 in BJ fibroblast cells (Fregoso, O., et al. (2013). Mole. Cell. 50:56-66).
Immunoprecipitation Analysis: A representative lot immunoprecipitated ASF-1 in BJ fibroblast cell lysate (Fregoso, O., et al. (2013). Mole. Cell. 50:56-66).
Research Category
Epigenetics & Nuclear Function
This Anti-ASF-1 Antibody, clone AK-96 is validated for use in western blotting, IP & ICC for the detection of ASF-1.

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected ASF-1 in 10 µg of HeLa cell lysate.

Replaces: MABE512

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

100

mouse

purified immunoglobulin

primary antibodies

AK-96, monoclonal

human

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

IgG2bκ

NP_001071634

Q07955

wet ice

~30 kDa observed

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1