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Anti-Amphiphysin II Antibody
| 产品编号： | 4104837 |
| 规格： | from rabbit, purified by affinity chromatography |
| 包装规格： | 100 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-Amphiphysin II Antibody
bridging integrator 1, amphiphysin-like, box dependant MYC interacting protein 1, Amphiphysin II, Box-dependent myc-interacting protein 1, Amphiphysin-like protein
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Amphiphysin is a nerve terminal-enriched protein thought to be involved in synaptic vesicle endocytosis. One of three amphiphysin homologs, amphiphysin II may interact with amphiphysin I in order to regulate synaptic vesicle endocytosis. During clathrin-mediated endocytosis, amphiphysin has been reported to bind to a component of the clathrin coat, clathrin assembly protein 2. Mutations in the expression of amphiphysin II may be causal to defects in the endocytosis pathway. Expression of amphiphysin II has been observed in neurons along with some endocrine cell types and spermatocytes. |
| Specificity【特异性】 | The antibody is raised against human amphiphysin II, but recognizes both amphiphysin I and Amphiphysin II (see Molecular Weight Notes). |
| Immunogen【免疫原】 | KLH-conjugated recombinant protein corresponding to human Amphiphysin II. Epitope: Unknown |
| Application【应用】 | Research Category Cell Structure Research Sub Category Cytoskeletal Signaling Western Blot Analysis: 1:1,000 dilution from a previus lot detected Amphiphysin II in 10 µg of rat brain and mouse brain membrane tissue lysates. Detect Amphiphysin II using this Anti-Amphiphysin II Antibody validated for use in WB. |
| Quality【质量】 | Evaluated by Western Blot in mouse brain tissue lysate. Western Blot Analysis: 1:1,000 dilution of this antibody detected Amphiphysin II in 10 µg of mouse brain tissue lysate. |
| Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. Affinity purified |
| Analysis Note【分析说明】 | Control Mouse brain tissue lysate |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | rat, mouse |
| species reactivity (predicted by homology) | human (based on 100% sequence homology) |
| technique(s) | western blot: suitable |
| isotype【同位素/亚型】 | IgG |
| NCBI accession no.【NCBI登记号】 | NP_004296 |
| UniProt accession no.【UniProt登记号】 | O00499 |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | It is well established (Ramjaun, 1997) that both Amphiphysin I and II run substantially slower on SDS-PAGE with a larger apparent molecular weight than the predicted molecular weights. |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
