Anti-Alanyl-tRNA synthetase (AARS Antibody), clone M6-P2E5

32160702

NA.41

Alanyl-tRNA synthetase (AARS), also known as Alanine-tRNA ligase, AlaRS, or Renal carcinoma antigen NY-REN-42, and encoded by the gene AARS, is a key transfer RNA synthetase responsible for the attachment of alanine to the tRNA(Ala) for protein translation. Alanine-tRNA ligase also can edit incorrectly charged tRNA(Ala) for proper translation. Alanine-tRNA ligase is a monomer and is localized in the cytoplasm and ubiquitously expressed. Mutations in Alanine-tRNA ligase have been linked to a progressive peripheral nervous disorder called Charcot-Marie Tooth disease. Moreover autoantibodies against aminoacyl tRNA synthetases are some of the most common autoantibodies detected in autoimmune diseases with nearly 25-35% of patients having them, and some of these such as autoantibodies to Alanine-tRNA ligase have also been associated with autologous antibodies in renal carcinoma patients.

Linear peptide corresponding to the C-terminus of human Alanyl-tRNA synthetase (AARS).

This Anti-Alanyl-tRNA synthetase (AARS Antibody), clone M6-P2E5 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Alanyl-tRNA synthetase (AARS).
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Alanyl-tRNA synthetase (AARS) in 10 µg of HepG2 cell lysate.
Immunohistochemistry Analysis: A representative lot detected Alanyl-tRNA synthetase (AARS) in human breast cancer tissue (Prof. Ayham Alnabulsi, University of Aberdeen)

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Alanyl-tRNA synthetase (AARS) in 10 µg of HeLa cell lysate.

Protein G Purified
Format: Purified

100

mouse

purified immunoglobulin

primary antibodies

P2E5, monoclonal

mouse, human

1 mg/mL

immunohistochemistry: suitable (paraffin)
western blot: suitable

IgG1κ

NP_001596

P49588

wet ice

~110 kDa observed

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1