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Anti-3-PGDH Antibody
| 产品编号： | 4104403 |
| 规格： | from rabbit, purified by affinity chromatography |
| 包装规格： | 100 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-3-PGDH Antibody
D-3-phosphoglycerate dehydrogenase, 3-PGDH
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | 3-PGDH (PHGDH) protein in an important enzyme in L-serine biosynthesis. 3-PGDH performs the first rate limiting step in the phosphorylated pathway of serine biosynthesis. 3-PGDH is also a biomarker in certain cancers that over express 3-PGDH. Moreover, 3-PGDH is the essential enzyme needed for cells to traverse the S phase and divide, and it plays a key role in T cell activation and proliferation for instance. Interestingly, even though 3-PGDH knockdown and reduced serine synthesis slows growth and proliferation of many cancer cells in vitro 3-PGDH knockdown does not affect tumor maintenance and growth in established breast cancer xenograft models, suggesting that 3-PGDH-dependent cancer cell growth may be context-dependent. |
| Immunogen【免疫原】 | Epitope: Near N-terminus KLH-conjugated linear peptide corresponding to human 3-PGDH near the N-terminus. |
| Application【应用】 | Research Category Signaling Research Sub Category Signaling Neuroscience This Anti-3-PGDH Antibody is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of 3-PGDH. Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected 3-PGDH in human cerebral cortex, human kidney, and human cerebellum tissue. |
| Quality【质量】 | Evaluated by Western Blotting in A431 cell lysate. Western Blotting Analysis: 0.2 µg/mL of this antibody detected 3-PGDH in 10 µg of A431 cell lysate. |
| Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Affinity purified |
| Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | human, mouse |
| species reactivity (predicted by homology) | chimpanzee (based on 100% sequence homology), porcine (based on 100% sequence homology) |
| technique(s) | immunohistochemistry: suitable (paraffin) western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NP_006614 |
| UniProt accession no.【UniProt登记号】 | O43175 |
| shipped in【运输】 | wet ice |
| Gene Information | human ... PHGDH(26227) |
产品说明
| Target description【目标描述】 | ~54 kDa observed |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
