Anti-Collagen Type I Antibody

32160702

NA.41

Recognizes Human collagen type I. Some cross reactivity with other proteins.

Human placental collagen type I

ELISA on human collagen type I: 1:4,000.

Indirect microimmunofluorescent visualization of collagen type I on cryostat sections of human tissue or cultured cells: 1:10-1:40.

Western blot 1:200

Not recommended for use in paraffin treated tissues.

Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
This Anti-Collagen Type I Antibody is validated for use in ELISA, IH, WB for the detection of Collagen Type I.
Research Sub Category
ECM Proteins

Format: Semi-Purified
Immunoglobulin fraction, prepared by ammonium sulfate precipitation and chromatography on DEAE-cellulose, of antiSerum cross-absorbed over immobilized human albumin, immunoglobulin and collagen types III, IV and V.

Product is liquid in 0.15M Sodium Chloride, 10mM Sodium Phosphate pH 7.5 with 0.1% Mannitol and 0.1% Dextran as stabilizers. No preservative.
Ammonium sulfater precipitation followed by DEAE chromatography, and immunodeletion

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

purified immunoglobulin (Semi-purified)

primary antibodies

polyclonal

human

Chemicon^®

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

not suitable for immunohistochemistry (Paraffin)

NP_000079

P02452

dry ice

human ... COL1A1(1277)

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2