Anti-Phosphotyrosine Antibody, clone 4G10^®, Magnetic Bead Conjugate

32160702

NA.41

Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.).The advent of anti-phosphotyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.

This antibody recognizes tyrosine-phosphorylated proteins from all species

Phosphotyramine-KLH

Detect Phosphotyrosine using this Anti-Phosphotyrosine Antibody, clone 4G10, Magnetic Bead Conjugate validated for use in IP.
Research Sub Category
General Post-translation Modification
Research Category
Signaling

100 μg of NIH3T3 whole cell lysate stimulated with 100 ng/mL PDGF (10 minutes) was immunoprecipitated (IP) using either anti-mouse IgG (Cat. No. 12-371) (lane 1), or Anti-Phosphotyrosine, Magnetic Bead Conjugate
(Lane 2).

20% slurry; 1xPBS, 0.05% sodium azide. Sufficient for 10 IPs.
Format: Purified
Protein G-Sepharose Chromatography

Control
NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes)

4G10 is a registered trademark of Upstate Group, Inc.

100

mouse

purified antibody

primary antibodies

4G10®, monoclonal

all

immunoprecipitation (IP): suitable

IgG2bκ

wet ice

Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.

Stable for 1 year at 2-8ºC from date of receipt.
Note: DO NOT FREEZE

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2