BIOshell^TM A400 Protein C4, 3.4 μm HPLC Column

SB.52

BIOshell^™ 400 Å Protein C4 columns contain 3.4 μm advanced Fused-Core particles with 400 Å pores that are specifically engineered to provide efficient separation of larger proteins. These columns provide excellent separations on very hydrophobic proteins such as those found in the cell membrane. The C4 ligand combined with an exhaustively endcapped surface ensures optimum protein recovery of larger or very hydrophobic biomolecules. The 3.4 μm Fused-Core particles allow a shorter analysis time yet similar back pressure to fully porous 1.7 μm, 300 Å fully porous C4 particles. These columns can be operated long term at pressures up to 9000 psi (600 bar), temperatures up to 90 ℃, and within the pH range of 2 to 9.

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

BIOshell is a trademark of sigma-aldrich Co. LLC

100

stainless steel hardware

suitable for USP L26

Shell thickness (0.2 μm)
Solid Core (3.0 μm)

BIOshell^™

endcapped

BIOshell^™

1 ea of

0.4% carbon loading

600 bar max. pressure
90 ℃ max. temp.

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

10 cm × 4.6 mm

4.2 μmol/m²

spherical silica particle platform
superficially porous particle

C4 (butyl) phase

3.4 μm

400 Å

15 m²/g

2 - 9

reversed phase