Ascentis^® Express 160 Å Phenyl-Hexyl, 2.7 μm HPLC Column

stainless steel column

suitable for USP L11

Ascentis^®

endcapped

1 ea of

4.7% Carbon loading

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

25 cm × 2.1 mm

Fused-Core^® particle platform
superficially porous particle

Phenyl-Hexyl (dimethylphenyl-hexylsilane) bonding phase

2.7 μm

160 Å pore size

90 m²/g

2 - 9

reversed phase

Ascentis^® Express 160 Å Phenyl-Hexyl is a high-speed, high-performance liquid chromatography column based on a 160Å Fused-Core^® particle design. The particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency for high molecular weight solutes (up to 20 kDa) due to the shallow diffusion paths in the 0.5 μm thick porous shell and the small overall particle size of 2.7 μm. The endcapped, phenyl-hexyl bonded phase provides a stable, reversed phase packing with a pore structure and pore size that is optimized for reversed-phase HPLC separations of peptides and polypeptides, using typical acidic mobile phases favored for proteomic applications.

Target analytes include electron-poor molecules, aromatic or unsaturated compounds (ketones, nitriles, alkenes), as well as peptides and polypeptides < 20 kDa.

Ascentis is a registered trademark of sigma-aldrich Co. LLC
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.