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Topoisomerase I human
| 产品编号： | 4096573 |
| 规格： | buffered aqueous glycerol solution, ≥2 units/μL |
| 包装规格： | 250 UNITS |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
基本信息
| Enzyme Commission (EC) Number【酶学委员会(EC)编号】 | 5.99.1.2 ( BRENDA | IUBMB ) |
| MDL number | MFCD03458765 |
| NACRES | NA.54 |
| General description【一般描述】 | Topoisomerase I (TOP1) is mapped to human chromosome 20q12. TOP1 is a 91 kDa type IB enzyme and consists of N-terminal highly charged domain, a core, linker domain and a C-terminal catalytic domain with Tyr-723 active site residue. |
| Application【应用】 | Topoisomerase I human has been used for Topoisomerase I assay for screening inhibitors. Topoisomerase I has been used in a study to assess implications for the regulation of HIV-1 replication. Topoisomerase I has also been used in a study to investigate the tumor suppressor protein kinase Chk2 is a mediator of anoikis of intestinal epithelial cells. |
| Biochem/physiol Actions【生化/生理作用】 | Topoisomerase I human (TOP1) interacts with DNA by wrapping and takes a bi-lobed conformation. TOPI is essential for normal development and transcriptional regulation. Variants in the TOP1 gene is observed in patients with colorectal cancer. Topoisomerase I relaxes supercoiled DNA molecules. The enzyme initiates transient breakages and rejoins of phosphodiester bonds in superhelical turns of closed-circular DNA. Enzyme activity is independent of right- and left-handed superhelices. |
| Unit Definition【单位定义】 | One unit will relax 0.25 μg of supercoiled plasmid DNA in 30 minutes at pH 7.9 at 37 ℃. |
| Physical form【外形】 | Solution containing 20 mM sodium phosphate, pH 7.4, 300 mM NaCl, 50 μg/mL BSA, 50% glycerol, and between 25-100 mM imidazole (concentration will be lot dependent). |
产品性质
| Quality Level【质量水平】 | 200 |
| Assay【测定】 | ≥2 units/μL |
| form【形式】 | buffered aqueous glycerol solution |
| mol wt【分子量】 | 100 kDa |
| concentration【浓度】 | ≥2 units/μL |
| UniProt accession no.【UniProt登记号】 | P11387 |
| storage temp.【储存温度】 | −70℃ |
| Gene Information | human ... TOP1(7150) |
安全信息
| Pictograms【象形图】 | GHS08 |
| Signal word【警示用语：】 | Danger |
| Hazard Statements | H360D |
| Precautionary Statements | P201 - P308 + P313 |
| Hazard Classifications【危险分类】 | Repr. 1B |
| Storage Class Code【储存分类代码】 | 6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects |
| WGK | WGK 1 |
