Anti-acetyl-Histone H3 (Lys9) Antibody, Trial Size

32160702

NA.41

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

This peptide sequence is identical in a wide range of animal and plant species.

KLH-conjugated, synthetic peptide (ARTKQTA RAcKSTG-C) corresponding to amino acids 1-12 of Histone H3.

Anti-acetyl-Histone H3 (Lys9) Antibody, Trial Size is a Rabbit Polyclonal Antibody for detection of Histone H3 acetylated on lysine 9 (H3K9ac). This purified Ab is specificity verified by dot blot (DB) and validated in WB, ChIP, ChIP-seq, FC. Small pack size is ideal for screening applications.
Western Blot Analysis:
A431, C2C12 lysate, HEK293 lysate, Hela
lysate, HepG2 lysate , Jurkat lysate , L6
lysate, and NIH/3T3 lysate were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-ANTI-ACETYL HIS H3 (1:500 dilution of a representative lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

Dot Blot Analysis: AbSurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665), which contain histone peptides with various modifications were probed with Cat. No. 06-942-S, Anti-acetyl-Histone H3 (Lys9) at a (1:500) dilution. Proteins were visualized using a Donkey anti-rabbit IgG conjugated to HRP and a chemiluminescence detection system.

Dot Blot Analysis:
0.5 μg/mL of a previous lot detected 50 ng of mono-acetylated peptide corresponding to position 9 in the N-terminal sequence of Histone H3 (Catalog # 12-358), but not the monoacetylated peptide corresponding to acetyl-lysine at position 14 (Catalog # 12-359) or unacetylated Histone H3 (Catalog # 12-357).

Immunoprecipitation:
Not determined.

Immunocytochemistry:
Not determined.

Routinely evaluated by Western Blot on HEK293 lysates.

Western Blot Analysis:
1:500 dilution of this lot detected ANTI-ACETYL HIS H3 on 10 μg of HEK293 lysates.

Purified rabbit IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.
Format: Purified
Protein A purified

Control
TSA-treated HeLa and NIH/3T3 cells.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

purified immunoglobulin

primary antibodies

polyclonal

eukaryotes, mouse, rat, human

Upstate^®

ChIP: suitable (ChIP-seq)
dot blot: suitable
western blot: suitable

IgG

NM_003493

Q16695

wet ice

human ... HIST1H3F(8968)

Approx. 17 kDa

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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WGK 1