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Anti-Caspase3 (active form) Antibody, clone 3D9.3
产品别名
Anti-Caspase3 (active form) Antibody, clone 3D9.3
Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, CPP-32, Protein Yama, SREBP cleavage activity 1, SCA-1, Caspase-3 subunit p17, Caspase-3 subunit p12
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Caspase-3 (CPP32, Apopain) is the most extensively studied apoptotic protein. Caspase-3 is synthesized as an inactive proenzyme (32 kDa) that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17 kDa) and small (12 kDa) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells such as PARP and DFF. |
| Specificity【特异性】 | This antibody demonstrates specificity for the cleaved 17 kDa active form, but does not cross-react with the non-active (pro) form Caspase3 at 35 kDa. |
| Immunogen【免疫原】 | KLH-conjugated linear peptide corresponding to human Caspase3 (active form). |
| Application【应用】 | Anti-Caspase3 (active form), clone 3D9.3 is an antibody targeting the Caspase3 (active form) protein, validated for use in WB, ICC & Flow. Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Caspase3 (active form) in Hela and Hela/staurosporine 3 hr cells. Flow Cytometry Analysis: A 1:400 dilution from a representative lot detected Caspase3 (active form) in Jurkat T cells. |
| Quality【质量】 | Evaluated by Western Blot in HeLa cell lysate. Western Blot Analysis: 0.1 µg/mL of this antibody detected Caspase3 (active form) in 10 µg of HeLa and staurosporine treated HeLa cell lysate. |
| Linkage【联系】 | Replaces: 04-1090; 04-439 |
| Physical form【外形】 | Purified by Ion-Exchange Chromatography Format: Purified |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| antibody form【抗体形式】 | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone【克隆】 | 3D9.3, monoclonal |
| species reactivity | human |
| technique(s) | flow cytometry: suitable immunocytochemistry: suitable western blot: suitable |
| isotype【同位素/亚型】 | IgMκ |
| NCBI accession no.【NCBI登记号】 | NP_004337 |
| UniProt accession no.【UniProt登记号】 | P42574 |
| shipped in【运输】 | wet ice |
| Gene Information | human ... CASP3(836) |
产品说明
| Target description【目标描述】 | ~17 kDa observed. Caspase3 is cleaved during apoptosis into 17 kDa and 12 kDa active forms. |
| 【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
