Anti-Tau phospho Serine 199 Antibody

32160702

NA.41

Tau is a neuronal microtubule-associated protein found predominantly on axons and functions to promote tubulin polymerization and stabilize microtubules. Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer′s disease (AD) brain. Hyperphosphorylated Tau is also found in neurofibrillary lesions in a range of other central nervous system disorders. Hyperphosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II (CK2), phosphorylate Tau. Phosphorylation of serine 199 is catalyzed by GSK-3- in vitro, and has been linked to frontotemporal dementia.

Tau phosphoSerine 199. The antibody recognizes Tau pSerine 199 in samples of recombinant human Tau treated with GSK-3beta for 45 minutes. The reactivity of the antibody is blocked with the pSerine 199 peptide but not the non-phosphopeptide or a generic phosphoSerine-containing peptide.

Synthetic peptide of amino acids surrounding the phosphoSerine 199 site of human Tau.

Research Category
Neuroscience
Western blot: 1:1,000. Suggested blocking buffer is 5% BSA-TBST for one hour at room temperature. Suggested antibody dilution buffer is 3% BSA-TBST. Suggested antibody incubation time is 2 hours at room temperature.

Optimal working dilutions must be determined by the end user.
Research Sub Category
Neurodegenerative Diseases
Anti-Tau phospho Serine 199 Antibody is an antibody against Tau phospho Serine 199 for use in WB.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

affinity purified immunoglobulin

primary antibodies

polyclonal

affinity chromatography

human

Chemicon^®

western blot: suitable

NM_005910.3
NM_016834.2
NM_016835.2
NM_016841.2

P10636

dry ice

Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self defrosting freezer.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 2