Anti-Tau Antibody, PAD, clone TNT-1

32160702

NA.41

Tau or Microtubule-associated protein tau (MAPT), also known as neurofibrillary tangle protein and paired helical filament-tau (PHF-tau), is a cytosolic protein that promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of axonal polarity in neurons. Tau binds to and is thought to function as a linker protein between axonal microtubules and neural plasma membrane components. There are multiple isoforms, and the short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. PAD is the phosphatase activating domain, and has been demonstrated to be involved in the inhibition of anterograde, kinesin-based fast axonal transport (FAT) by activating axonal protein phosphatase 1 (PP1) and glycogen synthase kinase 3 (GSK3), independent of microtubule binding. Defects in Tau are thought to be the cause of a number of neurodegenerative diseases, including frontotemporal dementia (FTD), pallido-ponto-nigral degeneration (PPND), Pick disease of the brain (PIDB), corticobasal degeneration (CBD), supranuclear palsy type 1 (PSNP1), Alzheimer disease, and Parkinson disease.

KLH-conjugated linear peptide corresponding to human Tau.

Anti-Tau, PAD, clone TNT-1 detects levels of Tau proteins & has been published & validated for use in WB, IHC, IF & ELISA.
Western Blot Analysis: 1 µg/mL from a representative lot detected Tau, PAD in 10 µg of human and mouse cortex tissue lysates.

Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Tau, PAD in human Alzheimer′s brain tissue.

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Tau, PAD in normal and Alzheimer′s tissues from entorhinal cortex, hippocampus, inferior temporal gyrus, and superior gyrus of control (Kanaan, N. M., et al. (2011). J Neurosci. 31(27):9858-9868.).

ELISA Analysis: A representative lot from an independent laboratory detected Tau, PAD in recombinant Tau protein (Kanaan, N. M., et al. (2011). J Neurosci. 31(27):9858-9868.).
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases

Evaluated by Western Blot in rat cortex tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Tau, PAD in 10 µg of rat cortex tissue lysate.

Replaces: MAB10417

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

Control
rat cortex tissue lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

100

mouse

purified antibody

primary antibodies

TNT-1, monoclonal

mouse, rat, human

ELISA: suitable
dot blot: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

IgG1κ

NP_001116538

P10636

wet ice

~55 kDa observed. Many isoforms are known to exist between 35-80 kDa An uncharacterized band may be observed in some cell lysates.

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1