Anti-Synuclein α Antibody

32160702

NA.41

alpha-Synuclein is a small (140 amino acid) cytoplasmic protein of unclear function that is enriched in presynaptic terminals and is the precursor protein of a nonamyloid beta component of senile plaques in Alzeheimer′s Disease (AD). α-Synuclein is located predominantly in the presynaptic nerve terminals in the brain but is also found in low concentrations in all tissues except liver. Recent studies have shown that abnormal aggregation and accumulation of synaptic proteins, including α-Synuclein might be associated with plaque formation in AD and seems to be a major component of Lewy bodies in dementia with Lewy bodies (DLB). It has also been identified in the Glial Cytoplasmic Inclusions (GCIs) found in Multiple System Atrophy (MSA).

Has not been tested on other species.
Alpha-synuclein. The immunizing peptide was chosen to reduce cross-reactivity of AB5038 with β-synuclein to a minimum. Immunohistochemical and Western blot analysis of human brain indicates a high level of specificity.

Human alpha-synuclein residue 111-131 coupled to KLH.
Epitope: a.a. 111-131

Immunohistochemistry:
1:1,000-1:2,000 dilutoin of a previous lot was used on paraffin sections; 1:4,000-1:12,000 dilution of a previous lot was used on frozen sections.

Western blot:
1:1,000-1:2,000. Shows a 14-19kDa band.

Immunocytochemistry:
Previous lot used IC.

Optimal working dilutions must be determined by end user.
Anti-Synuclein Antibody, α detects level of Synuclein & has been published & validated for use in IC, IH, IH(P) & WB.

Evaluated by Western Blot on Human Brain lysates.

Western Blot Analysis:
1:1000 dilution of this antibody detected Synuclein on 10 µg of human brain lysates.

Replaces: 04-1053

Rabbit polyclonal serum lyophilized, no preservatives. Reconstitute with 50 μL of sterile distilled water. Centrifuge to remove any residue.
Unpurified

Control
Human brain sections.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

serum

primary antibodies

polyclonal

rat, human

Chemicon^®

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

NM_000345.2

P37840

dry ice

human ... SNCA(6622)

~14-19 kDa

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10 - Combustible liquids

WGK 1