Anti-Macrophages/Granulocytes Antibody, clone OX-41

32160702

NA.41

Macrophages and granulocytes express Signal regulatory protein (SIRP), which is a transmembrane glycoprotein involved in the down regulation of tyrosine kinase receptor signal transduction induced by cell adhesion, growth factors or insulin. They are immunoglobulin-like cell surface receptor for CD47. By CD47 binding there is a negative regulation of phagocytosis, mast cell activation and dendritic cell activation that prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.

MAB1407P recognizes rat SIRP (Signal regulatory protein), which is expressed selectively by myeloid cells and by neurons. This monoclonal binds to a different epitope on SIRP than ED9. It does not block the interaction of CD172a and CD47.
Labels most macrophages including resident peritoneal, alveolar and activated macrophages. Recognition of other macrophages is limited to only occasional kupffer cells. In addition it labels granulocytes and a population of dendritic cells. It also labels brain extensively.
Expected to cross-react with rat and mouse.

Rat peritoneal macrophages.

Research Sub Category
Inflammation & Autoimmune Mechanisms
This Anti-Macrophages/Granulocytes Antibody, clone OX-41 is validated for use in FC, IC, IH, IH(P), WB for the detection of Macrophages/Granulocytes.
Immunohistochemistry:
Frozen sections and Formalin fixed, paraffin embedded tissue sections (requires trypsin or pronase pre-treatment).

Western Blotting:
A previous lot of this antibody was used in western blot.

Flow cytometry:
1:100-1:200 of a previous lot was used. (Use 10 μL of working dilution for 10^6 cells in 100 μL).

Optimal working dilutions must be determined by the end user.
Research Category
Inflammation & Immunology

Routinely evaluated by Immunocytochemistry on Macrophages/Granulocytes.

Immunocytochemistry Analysis:
Confolcal fluorescent analysis of Macrophages/ Granulocytes using anti-Macrophages/ Granulocytes mouse monoclonal antibody.

Replaces: MAB1407

ImmunoAffinity Purified
Purified mouse monoclonal IgG2a in buffer containing 0.09% sodium azide.

Control
Macrophages, monocytes, lymphoid organs.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

affinity isolated antibody

primary antibodies

OX-41, monoclonal

affinity chromatography

mouse, human, rat

Chemicon^®

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

IgG2a

NP_001035111

P78324

wet ice

human ... SIRPA(140885)

56 kDa

Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2