Donkey Anti-Rabbit IgG Antibody, HRP conjugate, Species Adsorbed

32160702

NA.46

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Rabbit IgG

Research Category
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
EIA and Western blots: 1:5,000-1:100,000.

Immunohistochemistry: 1:500-1:5,000.

Western blotting with ECL substrates: 1:10,000-1:200,000.

Optimal working dilutions must be determined by end user.
Donkey anti-Rabbit IgG Antibody, HRP conjugate, Species Adsorbed is an antibody against Donkey Rabbit IgG for use in ELISA & WB.

Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA. Contains no preservative.

RECONSTITUTION:

Reconstitute with 500 μL of sterile distilled water.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

donkey

100

peroxidase conjugate

affinity purified immunoglobulin

secondary antibodies

polyclonal

rabbit

Chemicon^®

ELISA: suitable
western blot: suitable

wet ice

Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

WARNING:

Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

11 - Combustible Solids

WGK 3