Anti-Rat Collagen Type I Antibody

32160702

NA.41

Collagen Type I extracted and purified from rat skin. Antibody shows less than 0.1% reactivity with human, mouse, chicken Collagen Type I, rat Collagen Types II, III and V and rat Elastin.

Recognizes Rat Collagen Type I
The antibody shows less than 0.1% reactivity with human, mouse, chicken Collagen Type I, rat Collagen Types II, III and V and rat Elastin.

Collagen Type I extracted and purified from rat skin.

ELISA:
A 1:200 dilution of a previous lot was used in ELISA.

RIA:
A previous lot of this antibody was used in RIA.

Immunocytochemistry:
A 1:40-1:80 dilution of a previous lot was used for immunofluorescent staining of frozen rat skin and liver tissues.
Can also be used for acetone-fixed cells.

Immunohistochemistry:
1:40-1:80 dilution for immunofluorescent staining of frozen rat skin and liver tissues. The antibody is also reactive on paraffin embedded rat tissues (skin, liver) at a tdilution of 1:500 using an ABC detection system.

Not recommended for Western blots.
Optimal working dilutions must be determined by the end user.
Anti-Rat Collagen Type I Antibody is an antibody against Collagen Type I for use in ELISA, IC, IH(P), RIA.

Immunohistochemistry(paraffin):
Collagen Type I (cat. # AB755P) staining pattern/morphology in rat skin. Tissue is pre-treated with Citrate pH 6.0, antigen retrieval. This lot of antibody is diluted to 1:1000, using IHC-Select reagents used with HRP-DAB. Immunoreactivity is seen fiber-staining on paraffin section. Note that the staining pattern is as expected.

IHC-Paraffin Staining With Epitope Retrieval: Rat Skin

Purified rabbit polyclonal. Contains no preservative.
ImmunoAffinity Purified

Control
Rat hepatic stellate cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

rat

Chemicon^®

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
radioimmunoassay: suitable

IgG

not suitable for Western blot

NM_000088.3

P02452

wet ice

human ... COL1A1(1277)

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

11 - Combustible Solids

WGK 1