Monoclonal Anti-p53-Biotin 小鼠抗

MFCD01864735

NA.41

p53 is a tumor suppressor gene expressed in a wide variety of tissues. It is a tetrameric nuclear DNA-binding phosphoprotein. The gene encoding it is localized on human chromosome 17p13.1 and mouse chromosome 11.

recombinant human wild type p53. The epitope resides beween amino acids 20-25 of human p53.

Monoclonal Anti-p53-Biotin antibody produced in mouse has been used for Western Blotting.

Tumor suppressor p53 has the capability to induce cell cycle arrest and has a role in DNA repair, senescence and apoptosis. It binds to Simian vacuolating virus 40 (SV40) T-antigen and human papilloma virus E6 protein. The p53 gene is mutated in various cancers, such as of the breast, ovarian, bladder, colon and lung.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

In order to obtain the best results using various techniques and preparations, we recommend determining optimal working dilutions by titration.

200

mouse

biotin conjugate

ascites fluid

primary antibodies

DO-1, monoclonal

buffered aqueous solution

antigen ~53 kDa

human

~1 mg/mL

flow cytometry: suitable
immunocytochemistry: 5-10 μg/mL using human A431
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 0.5-1 μg/mL using human A431 cell extracts

IgG2a

P04637

dry ice

−20℃

human ... TP53(7157)

For continuous use, store at 2-8℃ for up to one month.
For extended storage, freeze in working aliquots.
Repeated freezing and thawing, or storage in “frostfree”freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 3

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)