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Polynucleotide phosphorylase from Synechocystis sp.
产品别名
Polynucleotide phosphorylase from Synechocystis sp.
PNPASE
Polyribonucleotide Nucleotidyltransferase
基本信息
| Enzyme Commission (EC) Number【酶学委员会(EC)编号】 | 2.7.7.8 ( BRENDA | IUBMB ) |
| MDL number | MFCD00131992 |
| NACRES | NA.54 |
| General description【一般描述】 | Polynuclotide phosphorlyase in spinach chloroplasts acts as a exonuclease and a poly(A) polymerase. |
| Application【应用】 | Polynucleotide phosphorylase has been used in a study to discover that a major function of PNPase is the synthesis of CDP. It has also been used in a study to investigate the enzyme responsible for RNA 3′-tail synthesis in S. coelicolor. |
| Biochem/physiol Actions【生化/生理作用】 | Polynucleotide phosphorylase localizes to the intermembrane space of mitochondria and has a critical function in regulating mitochondrial homeostasis in human cells. Polynucleotide phosphorylase (PNPase) is a bifunctional enzyme with a phosphorolytic 3′ to 5′ exoribonuclease activity and a 3′-terminal oligonucleotide polymerase activity. |
| Unit Definition【单位定义】 | Supplied as a solution in 20 mM Hepes buffer pH 7.9, 0.1 mM EDTA, 2 mM DTT, 12.5 mM MgCl2, 60 mM KCl, 20% (w/v) Glycerol One unit will polymerize 1.0 μmole of ADP, releasing 1.0 μmole of inorganic phosphate in 15 minutes, at pH 9.1 at 37 ℃. |
产品性质
| Quality Level【质量水平】 | 200 |
| recombinant【重组】 | expressed in E. coli |
| description【描述】 | Histidine tagged |
| form【形式】 | solution |
| Specific Activity | ≥500 units/mg protein |
| mol wt【分子量】 | 85 kDa |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −70℃ |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
