Anti-ABCA4 Antibody, clone 3F4

32160702

Retinal-specific ATP-binding cassette transporter (UniProt F1MWM0/P78363; also known as ATP-binding cassette sub-family A member 4, ABC transporter A4, RIM ABC transporter, RIM Protein) is encoded by the ABCA4 gene in bovine species. ABC transporter A4, a ~250 kDa single-chain, is a multi-pass membrane protein of the superfamily of ATP-binding cassette (ABC) transporters that is localized to the disk margins of vertebrate photoreceptor outer segments. It is implicated in the clearance of all-trans retinal derivatives from rod and cone photoreceptor cells following photoexcitation as part of the visual cycle. ABC transporter A4 is composed of two symmetrically organized halves, each comprising six membrane-spanning helices, a large glycosylated exocytoplasmic domain located inside the disk, and a cytoplasmic domain with an ATP-binding cassette. Mutations in ABCA4 are known to cause Stargardt macular degeneration, which is an autosomal recessive form of juvenile macular degeneration associated with a severe loss in vision. (Ref.: Tsybovsky, Y et al. (2010). Adv. Exp. Med. Biol. 703, 105-125).

Clone 3F4 detects ABC transporter (ABCA4) in Bovine and Human cells. It targets an epitope within 9 amino acids from the C-terminal end.

Whole purified bovine disk rim protein.

Epitope: domain of:

Immunofluorescence Analysis: A 1:50 dilution from a representative lot detected ABCA4 in mouse retina cells.

Immunofluorescence Analysis: A 1:250 dilution from a representative lot detected ABCA4 in mouse retina cells (Courtesy of Dr. Robert Molday, Ph.D., University of British Columbia, Canada).

Immunoprecipitation Analysis: A representative lot detected ABCA4 in Immunoprecipitation applications (Bungert, S., et. al. (2001). J Biol Chem. 276(26):23539-46).

Western Blotting Analysis: A representative lot detected ABCA4 in Western Blotting applications (Molday, L.L., et. al. (2000). Nat Genet. 25(3):257-8; Illing, M., et. al. (1997). J Biol Chem. 272(15):10303-10; Beharry, S., et. al. (2004). J Biol Chem. 279(52):53972-9).

Immunohistochemistry Analysis: A representative lot detected ABCA4 in Immunohistochemistry applications (Molday, L.L., et. al. (2000). Nat Genet. 25(3):257-8).
Anti-ABCA4, clone 3F4, Cat. No. MABN2439, is a highly specific mouse monoclonal antibody that targets ABCA4 (RIM) and has been tested in Immunohistochemistry, Immunoprecipitation, Immunofluorescence, and Western Blotting.
Research Category
Neuroscience

Evaluated by Western Blotting in human retina tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected ABCA4 in 10 µg of human retinal tissue lysate.

Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Concentration: Please refer to lot specific datasheet.

100

mouse

purified immunoglobulin

primary antibodies

3F4, monoclonal

mouse, bovine, human

antibody small pack of 25 μg

immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

IgG1κ

F1MWM0

~220 kDa observed; 257.38 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1