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Cdk1/cyclin B Protein, active, 10 μg
产品编号: | 4094548 |
规格: | Active, C-terminal His6-tagged human full length Cdk1 & N-terminal GST-tagged human full length Cyclin B, for use in Kinase Assays. |
包装规格: | 10 μG |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
基本信息
eCl@ss | 32160405 |
NACRES | NA.26 |
General description【一般描述】 | Product Source: expressed individually by baculovirus in Sf21 insect cells C-terminal His6-tagged human full length cdk1, and N-terminal GST-tagged human full length cyclin B |
Biochem/physiol Actions【生化/生理作用】 | Protein Target: CDK1/cyclinB Target Sub-Family: CMGC |
Quality【质量】 | routinely evaluated by phosphorylation of the Histone H1 substrate |
Physical form【外形】 | Purified using Ni2+/NTA-agarose and GST-agarose, respectively. The cdk1 is then activated using CAK and repurified by Q Sepharose® and Ni2+/NTA-agarose |
Other Notes【其他说明】 | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
Legal Information【法律信息】 | Sepharose is a registered trademark of Cytiva UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | human |
mol wt【分子量】 | Mw 35 kDa (cdk1) Mw 75 kDa (cyclin B) |
manufacturer/tradename | Upstate® |
technique(s) | activity assay: suitable (kinase) |
NCBI accession no.【NCBI登记号】 | NM_001786.2 NM_033379.2 |
UniProt accession no.【UniProt登记号】 | P06493 |
packaging【包装】 | Also available in 250 µg size --call for pricing and availability and reference catalog number 14-450M when ordering the 250 µg size. |
产品说明
Storage and Stability【储存及稳定性】 | 6 months at -70°C |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |