PhosphoDetect Anti-PKR (pThr⁴⁵¹) Rabbit pAb

rabbit

100

primary antibodies

polyclonal

liquid

≤0.1% sodium azide as preservative

mouse, human

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

IgG

wet ice

−70℃

10 tests in Plastic ampoule

Recognizes the ~65-68 kDa PKR protein phosphorylated at Thr⁴⁵¹.
Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
This PhosphoDetect Anti-PKR (pThr⁴⁵¹) Rabbit pAb is validated for use in Immunoblotting, Immunohistochemistry for the detection of PKR (pThr⁴⁵¹).
Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phophorylation site, followed by immunoaffinity chromatography. Recognizes the ~65-68 kDa PKR protein phosphorylated at Thr⁴⁵¹.

a synthetic phosphopeptide corresponding to amino acids surrounding the Thr⁴⁵¹ phosphorylation site of human PKR (double-stranded RNA-dependent protein kinase)

Immunoblotting (1:1000)

Immunohistochemistry (see comments)

Toxicity: Standard Handling (A)

In Dulbecco′s PBS (without Mg⁺² or Ca⁺²), 0.1% BSA, 50% glycerol, pH 7.3.

Following initial thaw, aliquot and freeze (-70°C).

Positive Control
IFNγ-stimulated HeLa cells.

When used at a 1:1000 dilution there is sufficient antibody for 10 blots at 10 ml/blot. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the optimal dilution.
Cuddihy, A.R., et al.1999. Oncogene8, 2690.
Srivastava, S.P., et al. 1998. J. Biol. Chem.273, 2416.
Clemens, M.J. 1997. Int. J. Biochem. Cell Biol.29, 945.

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