Anti-Synaptophysin Antibody, clone SP15 (Ascites Free)

32160702

NA.41

Synaptophysin (UniProt P08247; also known as Major synaptic vesicle protein p38) is encoded by the SYP (also known as MRX96, MRXSYP) gene (Gene ID 6855) in human. Synaptophysin is a 38 kDa four-transmembrane glycoprotein on synaptic vesicle (SV) with both its N- and C-termini exposed cytoplasmically. Synaptophysin forms homomultimers in the SV membrane and is heavily phosphorylated on tyrosine residues in its C-terminal pentapeptide repeats. Synaptophysin is also known to interact with SV v-SNARE protein synaptobrevin II (sybII) when sybII is not engaged with other SNARE proteins, suggesting a role of synaptophysin in sybII retrieval during SV endocytosis. Consistently, synaptophysin-knockout neurons display reduced levels of sybII at their nerve terminals.

Human brain proteins immunoprecipitated with EP10 (Cat. No. MAB332).

This Anti-Synaptophysin Antibody, clone SP15 (Ascites Free) is validated for use in Western Blotting, Immunohistochemistry, Immunocytochemistry, ELISA for the detection of Synaptophysin.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Synaptophysin in 10 µg of human brain tissue lysate.
Western Blotting Analysis: A representative lot detected synaptophysin in rat hippocampal tissue extracts (Lin, D., et al. (2012). Behav. Brain Res. 228(2):319-327).
Western Blotting Analysis: Representative lots detected synaptophysin in mouse brain synaptosomes preparations (Shim, S.Y., et al. (2008). J. Neurosci. 28(14):3604-3614).
Western Blotting Analysis: A representative lot detected synaptophysin in the large dense-core vesicles-containing fractions obtained by sucrose gradient centrifugation of rat brain median eminence (ME) neuroterminals preparations (Yin, W., et al. (2007). Exp. Biol. Med. (Maywood). 232(5):662-673).
Immunohistochemistry Analysis: A representative lot detected a stronger synaptophysin immunoreactivity in the inner molecular layer of frozen dentate gyrus sections from a 3-week old monkey, while a stronger immunoreactivity was seen in the outer molecular layer of dentate gyrus sections from a 13-year old monkey (Lavenex, P., et al. (2007). Dev. Neurosci. 29(1-2):179–192).
Immunohistochemistry Analysis: A representative lot immunostained nerve terminals at palisade ending using cat extraocular muscle (EOM) whole mount sections (Konakci, K.Z., et al. (2005). Invest. Ophthalmol. Vis. Sci. 46(1):155-165).
Immunohistochemistry Analysis: A representative lot detected synaptophysin immunoreactivity in human brain tissue sections (Honer, W.G., et al. (1993). Brain Res. 609(1-2):9-20).
Immunocytochemistry Analysis: A representative lot detected punctate synatophysin immunoreactivity among cultured primary cerebrocortical neurons from rat embryos (Bragina, L., et al. (2006). J. Neurochem. 99(1):134-141.).
ELISA Analysis: Representative lots were employed as the capture antibody for the detection of synaptophysin in human temporal cortex, frontal cortex, and cerebellar cortex extracts by sandwich ELISA (Klucken, J., et al. (2006). Acta Neuropathol. 111(2):101-108; Fukumoto, H., et al. (2002). Arch. Neurol. 59(9):1381-1389).

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Synaptophysin in 10 µg of rat brain tissue lysate.

Purified by Ion-Exchange Chromatography
Purified mouse monoclonal IgMκ antibody in PBS with 0.05% sodium azide.
Format: Purified

Concentration: Please refer to lot specific datasheet.

100

mouse

purified antibody

primary antibodies

SP15, monoclonal

human, monkey, feline, mouse, rat

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

IgMκ

NP_003170

P08247

human ... SYP(6855)

~34 kDa observed

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2