Goat Anti-Mouse IgM μ chain Antibody, biotin conjugate, Species Adsorbed

32160702

NA.46

IgM constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as ′natural′ blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. The gene for the mu constant region contains four domains separated by short intervening sequences.

The antibody reacts with the heavy chain of mouse IgM as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.
Reacts with heavy chain of mouse IgM. Absorbed for mouse IgG1, IgG2a, IgG2b, IgG3 and IgA, pooled human sera and purified human paraproteins. Minimal cross reactivity with human immunoglobulins. Reactivity with other species has not been determined.

Epitope: IgM µ chain
Prepared from purified mouse IgM µ chain. Pooled antisera from goats hyperimmunized with mouse IgM paraproteins.

Research Category
Secondary & Control Antibodies
This Goat anti-Mouse IgM µ chain Antibody, biotin conjugate, Species Adsorbed is validated for use in ELISA for the detection of Mouse IgM µ chain.
ELISA: 1:5,000-1:20,000

Optimal working dilutions must be determined by the end user.
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling

Fragment Specific Secondary Antibodies

Purified by affinity chromatography on mouse IgM covalently linked to agarose. Liquid in 2.0 mL PBS/NaN₃.
Affinity Chromatography

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

goat

biotin conjugate

affinity purified immunoglobulin

secondary antibodies

polyclonal

mouse

Chemicon^®

0.5 mg/mL

ELISA: suitable

wet ice

Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 2