Goat Anti-Mouse IgM+IgG+IgA Antibody, F(ab′)2, biotin conjugate, Species Adsorbed

32160702

NA.46

IgG is the most abundant immunoglobulin and is comprised of two heavy chains and two light chains. Each molecule has two antigen binding sites. IgG has 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). IgM constitutes about 10% of serum immunoglobulins. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces.

Reacts with heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA. Absorbed for human sera and purified human paraproteins. Minimal cross reactivity with human immunoglobulins.
The antibody reacts with the heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.

Epitope: Mouse IgM, IgG, IgA
Prepared from pepsin digest of mouse Ig (H+L).

Goat anti-Mouse IgM+IgG+IgA Antibody, F(ab′)2, biotin conjugate, Species Adsorbed detects level of Mouse IgM+IgG+IgA & has been published & validated for use in ELISA & IF.
Research Sub Category
Fragment Specific Secondary Antibodies
Research Category
Secondary & Control Antibodies
Immunofluorescence: ≤ 1 μg/10⁶ cells.
ELISA: 1:5,000-1:20,000.

Optimal working dilutions must be determined by the end user.

Gel filtration chromatography
Purified by gel filtration chromatography of pepsin digested antibody. Liquid in 1.0 mL PBS/NaN₃.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

goat

biotin conjugate

purified antibody

secondary antibodies

polyclonal

mouse

Chemicon^®

0.5 mg/mL

ELISA: suitable
immunofluorescence: suitable

wet ice

Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt. Avoid repeated freeze/thaw cycles.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2