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Anti-Green Fluorescent Protein (26-39) Rabbit pAb
产品别名
Anti-Green Fluorescent Protein (26-39) Rabbit pAb
Anti-GFP
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | affinity isolated antibody |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
form【形式】 | liquid |
contains【包含】 | ≤0.1% sodium azide as preservative |
manufacturer/tradename | Calbiochem® |
storage condition【储存条件】 | OK to freeze avoid repeated freeze/thaw cycles |
isotype【同位素/亚型】 | IgG |
shipped in【运输】 | wet ice |
storage temp.【储存温度】 | −20℃ |
packaging【包装】 | 100 μL in Plastic ampoule Please refer to vial label for lot-specific concentration. |
基本信息
General description【一般描述】 | Anti-Green Fluorescent Protein (26-39), rabbit polyclonal, recognizes the ~28 kDa GFP and some GFP fusion proteins. It is validated for use in Western blotting and immunocytochemistry. Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~28 kDa GFP and some GFP fusion proteins. Recognizes the ~28 kDa GFP and some GFP fusion proteins. |
Immunogen【免疫原】 | Aequora victoria a synthetic peptide corresponding to amino acids 26-39 of Aequorea victoria wild-type green fluorescent protein |
Application【应用】 | Immunoblotting (1:2000) Immunocytochemistry (1:200) |
Warning【警告】 | Toxicity: Standard Handling (A) |
Physical form【外形】 | In PBS, 1 mg/ml BSA. |
Reconstitution【重悬】 | Following initial thaw, aliquot and freeze (-20°C). |
Other Notes【其他说明】 | This sequence is completely conserved between GFP and the modified and enhanced Blue, Green, and Yellow FP variants. This antibody has been successfully used in immunoblotting and immunocytochemistry protocols to detect GFP and fusion proteins. It detects a ~28 kDa protein that corresponds to GFP in immunoblots of protein extract from cells transfected with, and overexpressing, GFP isoforms. Antibody should be titrated for optimal results in individual sytems. Cubitt, A.B., et al. 1995. Trends Biochem. Sci.20, 448. |
Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 1 |