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PLH human cell line
产品性质
| biological source【生物来源】 | human blood |
| ethnicity【种族】 | Scandinavian |
| gender【性别】 | female |
| growth mode【生长模式】 | Suspension |
| karyotype【核型】 | Not specified |
| morphology【形态学】 | Lymphoblastoid |
| technique(s) | cell culture | mammalian: suitable |
| shipped in【运输】 | dry ice |
基本信息
| General description【一般描述】 | Consanguineous: Yes |
| Cell Line Description【细胞系描述】 | This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line. |
| DNA Profile【DNA图谱分析】 | A: 301 Cw: 602 B: 4701 HLA DRA: 101 HLA DRB1: 701 HLA DRB4: 101 HLA DQAP: 2.1 HLA DQA1: 201 HLA DQB1: 201 HLA DPA1: 1 HLA DPB1: 1501 |
| Culture Medium【培养基】 | RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%. |
| Subculture Routine【传代培养常规】 | Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 ℃. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium. |
| Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
产品说明
| Serological Profile【血清学分析】 | HLA A: 3 HLA A ief: 3.2 HLA C: 6 HLA B: 47 HLA B ief: 47 Bw4/bw6: 4 HLA Dw: DB1 HLA DR: 7 HLA DR53: 53 HLA DQ: 2 |
| Disclaimer【免责声明】 | This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations. |
