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Anti-phospho-STAT3 (Tyr705) Antibody, clone 9E12

产品编号:4085194
规格:clone 9E12, Upstate®, from mouse
包装规格:100 μg
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4085194100 μg5900
产品别名

Anti-phospho-STAT3 (Tyr705) Antibody, clone 9E12

Acute-phase response factor, DNA-binding protein APRF, signal transducer and activator of transcription 3, signal transducer and activator of transcription 3 (acute-phase response factor)

基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
STATs (signal transducers and activators of transcription) were originally discovered as two proteins (STAT1 and STAT2) which were involved in interferon-alpha (IFN-alpha) and IFN-gamma signal transduction. Since then, several additional STAT proteins have been identified (STAT3, 4, 5a, 5b, and 6). STATs undergo tyrosine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. In some cases this process is mediated by JAK Kinases (Janus Kinases 1, 2, and 3). For maximum activation of these proteins, phosphorylation at specific tyrosine and serine residues may be required in STAT1 alpha, 3, 4, and 5. Specific functions of the various members of the STAT family are poorly understood. STAT3 has been shown to be activated by IFN-alpha but not IFN-beta. The transcription factors associated with STAT3 are c-Jun and cyclic AMP-responsive enhancer binding protein (CREB). Deletion of the STAT3 gene in knock-out mice was lethal at the early embryonic stage.
Specificity【特异性】
This antibody recognizes STAT3, Mr 92 kDa, phos-phorylated on tyrosine-705. Clone 9E12.
Immunogen【免疫原】
KLH-conjugated phosphopeptide containing the AP[pY]LK motif of STAT3
Application【应用】
Immunoprecipitation: An independent laboratory has reported 1-10 µg/mL of a previous lot of this antibody immunoprecipitated phospho-STAT3 (Tyr705) from a lysate of 106 vanadate-treated human A431 cells.

ELISA: 0.05 µg/mL of this antibody is recommended by an independent lab for ELISA testing. Test was conducted on a previous lot.
Research Sub Category
Transcription Factors
Use Anti-phospho-STAT3 (Tyr705) Antibody, clone 9E12 (Mouse Monoclonal Antibody) validated in ELISA, IP, WB to detect phospho-STAT3 (Tyr705) also known as Acute-phase response factor or DNA-binding protein APRF. This Stat3 Antibody has been validated in Human, Mouse and Canine samples.
Research Category
Epigenetics & Nuclear Function
Quality【质量】
Routinely evaluated by immunoblot on lysates from human SK-N-MC cells stimulated with CNTF.

Western Blot Analysis:
0.1-1 µg/mL of this lot detected phospho-STAT3 (Tyr705) in lysates from human SK-NMC cells stimulated with CNTF.
Linkage【联系】
Replaces: 04-1059
Physical form【外形】
Format: Purified
Thiophilic adsoption and size exclusion chromatography
Lyophilized purified mouse IgG1ќ from 1 mL 2x PBS / 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 100 µL H2O (15 min, RT) for a final concentration of 1 mg/mL.
Analysis Note【分析说明】
Control
SK-N-MC cells

murine macrophage 4/4 cells, serum-starved for 16 hours then stimulated with Phorbol 12-myristate 13-acetate (PMA) for 10 minutes
Other Notes【其他说明】
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information【法律信息】
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
Quality Level【质量水平】
100
biological source【生物来源】
mouse
antibody form【抗体形式】
purified immunoglobulin
antibody product type
primary antibodies
clone【克隆】
9E12, monoclonal
species reactivity
mouse, canine, human
manufacturer/tradename
Upstate®
technique(s)
ELISA: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype【同位素/亚型】
IgG1κ
NCBI accession no.【NCBI登记号】
NM_139276
UniProt accession no.【UniProt登记号】
P40763
shipped in【运输】
dry ice
Gene Information
human ... STAT3(6774)
产品说明
Target description【目标描述】
92 kDa
Storage and Stability【储存及稳定性】
Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Pictograms【象形图】
GHS06
Signal word【警示用语:】
Danger
Hazard Statements
H302 + H332 - H311 - H412
Precautionary Statements
P261 - P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312
Hazard Classifications【危险分类】
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3
Storage Class Code【储存分类代码】
6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 1
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