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Anti-Paxillin Antibody, clone 5H11

产品编号:4085175
规格:clone 5H11, Upstate®, from mouse
包装规格:250 μg
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4085175250 μg6190
产品别名

Anti-Paxillin Antibody, clone 5H11

FLJ16691, paxillin

基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
Paxillin, a focal adhesion scaffolding protein, is phosphorylated on multiple tyrosine residues in response various stimulations including integrin stimulation, growth factor stimulation, and oncogenic transformation (v-Src, v-Crk and BCR/ABL). Paxillin binds many other focal adhesion proteins including FAK, Vinculin, Talin, and Tensin. As such, Paxillin and it tyrosine phosphorylation, help regulate the dynamics of the focal adhesions and the actin cytoskeleton which results in cell morphology and cell migration characteristics of the cell. Paxillin is most notable phosphorylated on tyrosines 31 and 118 (Tyr31 and Tyr118). Interestingly, Vinculin and FAK bind to overlapping sites on Paxillin. Additionally, there is an increase in Paxillin phosphorylation at Tyr118 in vinculin (-/-) and Vinculin Y822F cells. Paxillin Y31F Y118F expression restores apoptosis and inhibits Erk activity.
Specificity【特异性】
Other species cross-reactivity not tested.
This antibody recognizes paxillin.
Immunogen【免疫原】
GST fusion protein containing fulllength human paxillin. Clone 5H11.
Application【应用】
Research Sub Category
Cytoskeletal Signaling
Immunoprecipitation:
4 μg of a previous lot of antibody immunoprecipitated paxillin from 500 μg of a 3T3/A31 RIPA lysate.

Immunocytochemistry:
5-10 μg/mL of a previous lot gave positive immunostaining for paxillin in REF (rat embryo fibroblast) 52 cells fixed with 3.7% paraformaldehyde for 5 minutes.
Anti-Paxillin Antibody, clone 5H11 is an antibody against Paxillin for use in IC, IP & WB.
Research Category
Signaling
Quality【质量】
Routinely evaluated by Western Blot on RIPA lysates from 3T3/A31 fibroblasts.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected paxillin in RIPA lysates from 3T3/A31 fibroblasts.
Linkage【联系】
Replaces: 04-581
Physical form【外形】
Purified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide. Store at -20°C.
Format: Purified
Protein G Purified
Analysis Note【分析说明】
Control
Included Positive Antigen Control:
Catalog # 12-305, 3T3/A31 cell lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Other Notes【其他说明】
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information【法律信息】
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
Quality Level【质量水平】
100
biological source【生物来源】
mouse
antibody form【抗体形式】
purified immunoglobulin
antibody product type
primary antibodies
clone【克隆】
5H11, monoclonal
species reactivity
avian, rat, mouse, human, chicken, bovine
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype【同位素/亚型】
IgG1
NCBI accession no.【NCBI登记号】
NM_002859
UniProt accession no.【UniProt登记号】
P49023
shipped in【运输】
dry ice
产品说明
Target description【目标描述】
68 kDa
Storage and Stability【储存及稳定性】
Stable for 1 year at -20ºC from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 1
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