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Anti-Paxillin Antibody, clone 5H11
产品别名
Anti-Paxillin Antibody, clone 5H11
FLJ16691, paxillin
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | Paxillin, a focal adhesion scaffolding protein, is phosphorylated on multiple tyrosine residues in response various stimulations including integrin stimulation, growth factor stimulation, and oncogenic transformation (v-Src, v-Crk and BCR/ABL). Paxillin binds many other focal adhesion proteins including FAK, Vinculin, Talin, and Tensin. As such, Paxillin and it tyrosine phosphorylation, help regulate the dynamics of the focal adhesions and the actin cytoskeleton which results in cell morphology and cell migration characteristics of the cell. Paxillin is most notable phosphorylated on tyrosines 31 and 118 (Tyr31 and Tyr118). Interestingly, Vinculin and FAK bind to overlapping sites on Paxillin. Additionally, there is an increase in Paxillin phosphorylation at Tyr118 in vinculin (-/-) and Vinculin Y822F cells. Paxillin Y31F Y118F expression restores apoptosis and inhibits Erk activity. |
Specificity【特异性】 | Other species cross-reactivity not tested. This antibody recognizes paxillin. |
Immunogen【免疫原】 | GST fusion protein containing fulllength human paxillin. Clone 5H11. |
Application【应用】 | Research Sub Category Cytoskeletal Signaling Immunoprecipitation: 4 μg of a previous lot of antibody immunoprecipitated paxillin from 500 μg of a 3T3/A31 RIPA lysate. Immunocytochemistry: 5-10 μg/mL of a previous lot gave positive immunostaining for paxillin in REF (rat embryo fibroblast) 52 cells fixed with 3.7% paraformaldehyde for 5 minutes. Anti-Paxillin Antibody, clone 5H11 is an antibody against Paxillin for use in IC, IP & WB. Research Category Signaling |
Quality【质量】 | Routinely evaluated by Western Blot on RIPA lysates from 3T3/A31 fibroblasts. Western Blot Analysis: 0.5-2 μg/mL of this lot detected paxillin in RIPA lysates from 3T3/A31 fibroblasts. |
Linkage【联系】 | Replaces: 04-581 |
Physical form【外形】 | Purified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide. Store at -20°C. Format: Purified Protein G Purified |
Analysis Note【分析说明】 | Control Included Positive Antigen Control: Catalog # 12-305, 3T3/A31 cell lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels. |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
Legal Information【法律信息】 | UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified immunoglobulin |
antibody product type | primary antibodies |
clone【克隆】 | 5H11, monoclonal |
species reactivity | avian, rat, mouse, human, chicken, bovine |
manufacturer/tradename | Upstate® |
technique(s) | immunocytochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
isotype【同位素/亚型】 | IgG1 |
NCBI accession no.【NCBI登记号】 | NM_002859 |
UniProt accession no.【UniProt登记号】 | P49023 |
shipped in【运输】 | dry ice |
产品说明
Target description【目标描述】 | 68 kDa |
Storage and Stability【储存及稳定性】 | Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |