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MMP-2, Proenzyme, Human Rheumatoid Synovial Fibroblast

产品编号:4083904
包装规格:5 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
40839045 μG3340
产品别名

MMP-2, Proenzyme, Human Rheumatoid Synovial Fibroblast

Matrix Metalloproteinase 2, Gelatinase A, 72 kDa Type IV Collagenase

基本信息
Enzyme Commission (EC) Number【酶学委员会(EC)编号】
3.4.24.24 ( BRENDA | IUBMB )
General description【一般描述】
Native pro-MMP-2 from cultured, human rheumatoid synovial fibroblasts. Highly purified native enzyme derived from cell culture. Secreted by several cell types. Exists in a stable, but non-covalent 1:1 complex with TIMP-2. Requires activation prior to use.
Note: 1 mU = 1 milliunit.
Native pro-MMP-2 from cultured, human rheumatoid synovial fibroblasts. Secreted by several cell types. Requires activation just prior to use.
Warning【警告】
Toxicity: Standard Handling (A)
Unit Definition【单位定义】
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C, pH 7.0.
Physical form【外形】
In 200 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% BRIJ® 35 Detergent, 0.05% NaN₃, pH 7.0.
Preparation Note【制备说明】
Prepared from culture medium of human rheumatoid synovial fibroblasts that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Reconstitution【重悬】
Following initial thaw, aliquot and freeze (-70°C).
Other Notes【其他说明】
Buisson-Leqendre, N., et al. 2004. Arth. Rheumat.50, 2151.
Morgunova, E., et al. 2002. Proc. Natl. Acad. Sci. USA99, 7414.
Kolkenbrock, H., et al. 1997. Biol. Chem. 378, 71.
Legal Information【法律信息】
Brij is a registered trademark of Croda International PLC
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
Assay【测定】
≥90% (SDS-PAGE)
Quality Level【质量水平】
100
form【形式】
liquid
Specific Activity
≥600 mU/mg protein
manufacturer/tradename
Calbiochem®
storage condition【储存条件】
OK to freeze
avoid repeated freeze/thaw cycles
solubility【溶解性】
aqueous buffer: soluble
foreign activity【异质活性】
other MMP activity, none detected
shipped in【运输】
wet ice
storage temp.【储存温度】
−70℃
packaging【包装】
5 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.
安全信息
Storage Class Code【储存分类代码】
11 - Combustible Solids
WGK
WGK 1
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