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MMP-2, Proenzyme, Human Rheumatoid Synovial Fibroblast
产品别名
MMP-2, Proenzyme, Human Rheumatoid Synovial Fibroblast
Matrix Metalloproteinase 2, Gelatinase A, 72 kDa Type IV Collagenase
基本信息
Enzyme Commission (EC) Number【酶学委员会(EC)编号】 | 3.4.24.24 ( BRENDA | IUBMB ) |
General description【一般描述】 | Native pro-MMP-2 from cultured, human rheumatoid synovial fibroblasts. Highly purified native enzyme derived from cell culture. Secreted by several cell types. Exists in a stable, but non-covalent 1:1 complex with TIMP-2. Requires activation prior to use. Note: 1 mU = 1 milliunit. Native pro-MMP-2 from cultured, human rheumatoid synovial fibroblasts. Secreted by several cell types. Requires activation just prior to use. |
Warning【警告】 | Toxicity: Standard Handling (A) |
Unit Definition【单位定义】 | One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C, pH 7.0. |
Physical form【外形】 | In 200 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% BRIJ® 35 Detergent, 0.05% NaN₃, pH 7.0. |
Preparation Note【制备说明】 | Prepared from culture medium of human rheumatoid synovial fibroblasts that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV. |
Reconstitution【重悬】 | Following initial thaw, aliquot and freeze (-70°C). |
Other Notes【其他说明】 | Buisson-Leqendre, N., et al. 2004. Arth. Rheumat.50, 2151. Morgunova, E., et al. 2002. Proc. Natl. Acad. Sci. USA99, 7414. Kolkenbrock, H., et al. 1997. Biol. Chem. 378, 71. |
Legal Information【法律信息】 | Brij is a registered trademark of Croda International PLC CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
Assay【测定】 | ≥90% (SDS-PAGE) |
Quality Level【质量水平】 | 100 |
form【形式】 | liquid |
Specific Activity | ≥600 mU/mg protein |
manufacturer/tradename | Calbiochem® |
storage condition【储存条件】 | OK to freeze avoid repeated freeze/thaw cycles |
solubility【溶解性】 | aqueous buffer: soluble |
foreign activity【异质活性】 | other MMP activity, none detected |
shipped in【运输】 | wet ice |
storage temp.【储存温度】 | −70℃ |
packaging【包装】 | 5 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. |
安全信息
Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
WGK | WGK 1 |