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Anti-RPA2 p34 Antibody, clone RPA20 1-46
产品别名
Anti-RPA2 p34 Antibody, clone RPA20 1-46
RF-A protein 2, RP-A p32, RP-A p34, Replication factor A protein 2, replication protein A2 (32kD), replication protein A2, 32kDa, Replication protein A 32kDa subunit, RF-A, replication factor-A protein 2, p32, p34, RPA2, REPA2, RPA32.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | RPA is a heterotrimeric protein complex that binds specifically to single-stranded DNA (ssDNA). It is composed of three subunits, RPA1 (70 kDa), RPA2 (32 kDa), and RPA3 (14 kDa), and plays multiple roles in DNA metabolism. RPA is required for DNA replication initiation, as well as replication elongation. At the onset of DNA replication, RPA is loaded onto chromatin after the binding of Cdc45 to origins. RPA is needed for subsequent loading of DNA polymerase and other replication proteins to initiate DNA replication. After DNA replication begins, RPA moves with replication forks, stabilizing ssDNA and assisting in DNA synthesis. In addition to its replication function, RPA is also known to play essential roles in damage repair and recombination.The 32 kDa subunit, is phosphorylated by the cdc2 family of kinases when cells enter S-phase and in response to DNA damage by ATM, ATR, and DNA-PK. Alternate names for RPA32 include replication protein A 32 kDa subunit, RP-A, RF-A, replication factor-A protein 2, p32, p34, RPA2, REPA2, and RPA32. |
| Specificity【特异性】 | This antibody recognizes RPA2. |
| Immunogen【免疫原】 | His-tagged recombinant protein corresponding to human RPA2. Epitope: Unknown |
| Application【应用】 | Research Sub Category Cell Cycle, DNA Replication & Repair Chromatin Biology Anti-RPA2 p34 Antibody, clone RPA20 1-46 is a Mouse Monoclonal Antibody for detection of RPA2 p34 also known as Replication factor A protein 2 & has been validated in WB. Western Blot (SNAP ID) Analysis: 0.5 µg/mL from a previous lot detected RPA2 on 10 µg of NIH/3T3 cell lysate. Research Category Epigenetics & Nuclear Function |
| Quality【质量】 | Evaluated by Western Blot in HeLa cell lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected RPA2 on 10 µg of HeLa cell lysate. |
| Physical form【外形】 | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. Format: Purified Protein G Purified |
| Analysis Note【分析说明】 | Control HeLa cell lysate |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | 1-46, monoclonal RPA20, monoclonal |
| species reactivity | mouse, human |
| packaging【包装】 | antibody small pack of 25 μg |
| technique(s) | western blot: suitable |
| isotype【同位素/亚型】 | IgG1κ |
| NCBI accession no.【NCBI登记号】 | NM_002946 |
| UniProt accession no.【UniProt登记号】 | P15927 |
| shipped in【运输】 | ambient |
产品说明
| Target description【目标描述】 | ~32 kDa |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
