Anti-TSTA3 Antibody, clone 2N10-H1 ZooMAb^® Rabbit Monoclonal

rabbit

200

expressed in HEK 293 cells

unconjugated

purified antibody

primary antibodies

2N10-H1, monoclonal
recombinant monoclonal

ZooMAb^® learn more

lyophilized

calculated mol wt ~ 35.89 kDa
observed mol wt ~ 38 kDa

human

antibody small pack of 25 μL

recombinant expression
Learn more about Antibody Enhanced Validation

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

IgG

ambient

2-8℃

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Clone 2N10-H1 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects human GDP-L-fucose synthase. It targets an epitope within 20 amino acids from the C-terminal region.

KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of human GDP-L-fucose synthase.

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected TSTA3 in HeLa cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected TSTA3 in MCF-7 cell lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100-1,000 dilution from a representative lot detected TSTA3 in human testis and human kidney tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected TSTA3 in HepG2 cells.

Affinity Binding Assay: A representative lot of this antibody bound TSTA3 peptide with a KD of 1.7 x 10-8 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-TSTA3, clone 2N10-H1 ZooMAb^®, Cat. No. ZRB1198, is a recombinant Rabbit monoclonal antibody that specifically targets TSTA3 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

GDP-L-fucose synthase (UniProt: Q13630; also known as EC:1.1.1.271, GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase, Protein FX, Red cell NADP(H)-binding protein, Short-chain dehydrogenase/reductase family 4E member 1) is encoded by the GFUS (also known as SDR4E1, TSTA3) gene (Gene ID: 7264) in human. In mammals, fucosylated glycans play important roles in ABO blood group antigens, host-microbe interactions, and several human diseases, including rheumatoid arthritis, pancreatic cancer, and hepatocellular carcinoma. GDP-L-fucose synthase is a homodimeric protein of the NAD(P)-dependent epimerase/dehydratase family that is responsible for the last step of the major metabolic pathway resulting in GDP-L-fucose synthesis from GDP-D-mannose. It catalyzes a combined epimerase and NADPH-dependent reductase reaction, converting GDP-4-keto-6-D-deoxymannose to GDP-L-fucose. This reaction involves epimerization at both C-3 and C-5 followed by an NADPH-dependent reduction of the carbonyl at C-4. GDP-L-fucose synthase is also reported to act as the main autoantigen for a brain-infiltrating T cell clone. It is recognized by cerebrospinal fluid-infiltrating CD4+ T cells from HLA-DRB3*-positive subjects. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Santos, R., et al. (2018). Sci. Trans. Med. 10(462); eaat4301; Zhou, H., et al. (2013). Acta Biochim. Biophys. Sinica. 45(9); 720-725).

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

11 - Combustible Solids

WGK 1