Anti-RNA polymerase II subunit B1 Antibody, clone 4F8

32160702

NA.41

RNA polymerase II subunit B1 (RPB1) is the largest subunit of the RNA polymerase II complex. As a holoenzyme RNA polymerase II catalyzes transcription of eukaryotic DNA into RNA using the four ribonucleoside triphosphates as substrates. The RBP1 subunit, in combination with other polymerase subunits, forms a large central cleft that maintains contact between the active site of the enzyme, the DNA template, and the nascent RNA transcript. This subunit also contains a carboxy terminal domain (CTD) consisting of tandem heptapeptide repeats. In actively transcribing RNA polymerase ‘Ser-2’ and ‘Ser-5’ of the heptapeptide repeat are phosphorylated. Phosphorylation activates the RNA polymerase II beta subunit, allowing it to serve as an assembly platform for additional subunits that modulate initiation, elongation, termination and mRNA processing.

This antibody recognizes RNA polymerase II subunit B1.

Ovalbumin-conjugated linear peptide corresponding to human RNA polymerase II subunit B1.
Epitope: Unknown

Anti-RNA polymerase II subunit B1 Antibody, clone 4F8 is a Rat monoclonal antibody for detection of RNA polymerase II subunit B1 also known as DNA-directed RNA polymerase II A & has been validated in WB, ELISA.
Research Sub Category
Transcription Factors

RNA Metabolism & Binding Proteins
Chromatin Immunoprecipitation Analysis: A representative lot was used by independent laboraotry in ChIP. (Chapman, R., et al. (2007). Science. 318(5857):1780 -1782.)
Research Category
Epigenetics & Nuclear Function

Epigenetics & Nuclear Function

Evaluated by Western Blot in NIH/3T3 cell lysates.

Western Blot Analysis: 1 µg/ml of this antibody detected RNA polymerase II subunit B1 on 10 µg of NIH/3T3 cell lysates.

Purified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Format: Purified
Protein G Purified

Control
NIH/3T3 cell lysates

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

100

rat

purified antibody

primary antibodies

4F8, monoclonal

mouse

human (based on 100% sequence homology)

ChIP: suitable
ELISA: suitable
western blot: suitable

IgG1κ

NP_000928

P24928

wet ice

~ 220 kDa

Stable for 1 year at 2-8°C from date of receipt.

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WGK 1