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Anti-ROKα/ROCK-II Antibody, clone A9W4, rabbit monoclonal
| 产品编号： | 4082526 |
| 规格： | culture supernatant, clone A9W4, from rabbit |
| 包装规格： | 200 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-ROKα/ROCK-II Antibody, clone A9W4, rabbit monoclonal
RhoA-binding kinase 2, Rho-associated, coiled-coil containing protein kinase 2, Rho-associated, coiled-coil-containing protein kinase II, p150 ROK-alpha, p164 ROCK-2, ROCK-II, ROKalpha, Rock2
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Rho-Associated Kinase (Rho-Kinase or ROK) has been classified into two isoforms: ROKα/ROCK-II and ROKβ/ROCK-I. These large cellular proteins (150-160 kDa), have been reported to be involved in the formation of stress fibers, focal adhesions and cardiac hypertonic responses. Current research suggests that ROK of smooth muscle may be involved in the contractile process via phosphorylation of MYPT1 and Myosin. |
| Specificity【特异性】 | Recognizes ROKα/ROCK-II, Mr 150 kDa. Does not recognize ROKβ/ROCK-I. A non-specific band may also be detected, Mr 80 kDa. Predicted to cross-react with mouse and bovine based on sequence homology. |
| Immunogen【免疫原】 | 6His-tagged fusion protein corresponding to residues 2-543 of rat ROKα/ROCK-II. |
| Application【应用】 | Detect ROKα/ROCK-II using this Anti-ROKα/ROCK-II Antibody, clone A9W4 validated for use in IP & WB. Research Sub Category Cytoskeletal Signaling Research Category Signaling Immunoprecipitation: 4 μL of a previous lot immunoprecipitated ROKα/ROCK-II from 500 μg of PC-12 lysate. |
| Quality【质量】 | Routinely evaluated by Western BLot on RIPA lysates from PC-12 cells. Western Blot Analysis: A 1:500-1:5000 dilution of this antibody detected ROKα/ROCK-II in RIPA lysates from PC-12 cells. |
| Linkage【联系】 | Replaces: 05-841 |
| Physical form【外形】 | Cultured supernatant containing 0.05% sodium azide. |
| Analysis Note【分析说明】 | Control RIPA lysates from PC-12 cells |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | culture supernatant |
| antibody product type | primary antibodies |
| clone【克隆】 | A9W4, monoclonal |
| species reactivity | mouse, human, bovine, rat |
| technique(s) | immunoprecipitation (IP): suitable western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NM_013022 |
| UniProt accession no.【UniProt登记号】 | Q62868 |
| shipped in【运输】 | dry ice |
产品说明
| Target description【目标描述】 | 150 kDa |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
