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HMS174(DE3)pLysS Competent Cells-Novagen
| 产品编号： | 4082125 |
| 规格： | HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. T7 lysozyme expression suppresses basal T7 expression. |
| 包装规格： | 1 ML |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品性质
| Quality Level【质量水平】 | 100 |
| manufacturer/tradename | Novagen® |
| storage condition【储存条件】 | OK to freeze avoid repeated freeze/thaw cycles |
| cell transformation【细胞转化】 | transformation efficiency: >5×106 cfu/μg |
| storage temp.【储存温度】 | −70℃ |
| packaging【包装】 | 0.4 ml in Plastic ampoule 1 ml in Glass bottle |
基本信息
| General description【一般描述】 | HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. T7 lysozyme expression suppresses basal T7 expression. HMS174 strains provide the recA mutation in aK-12 background. Like BLR, these strains maystabilize certain target genes whose productsmay cause the loss of the DE3 prophage. DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability. Genotype: F-recA1 hsdR(rK12- mK12+) (DE3) pLysS (CamR, Rif R) This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form. | ||||||||||||||||
| Components【组分】 | | 0.4 ml | 1 ml | Component | • | 2 × 0.2 ml | 5 × 0.2 ml | HMS174(DE3)pLysS Competent Cells | • | 2 × 2 ml | 4 × 2 ml | SOC Medium | • | 2 ng | 2 ng | Test Plasmid | | |
| Warning【警告】 | Toxicity: Multiple Toxicity Values, refer to MSDS (O) | ||||||||||||||||
| Legal Information【法律信息】 | NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 2 |
