Anti-Cone Arrestin Antibody

32160702

NA.41

Arrestin-C (UniProt: Q9EQP6; also known as Cone arrestin, cArr, Retinal cone arrestin-3) is encoded by the Arr3 gene (Gene ID: 170735) in murine species. Arrestins are a superfamily of multi-functional proteins that that regulate signaling and trafficking of the majority of G protein-coupled receptors (GPCRs), as well as sub-cellular localization and activity of many other signaling proteins. Arrestin-C is predominantly found in Inner and outer segments, and the inner plexiform regions of the retina. It is expressed in cone photoreceptors and pinealocytes and may contribute to the shut-off mechanisms associated with high acuity color vision. Arrestin-C is an elongated two-domain molecule with overall fold and key inter-domain interactions that hold the free protein in the basal conformation similar to the other subtypes. Several structural elements are reported to contribute to arrestin binding. The C-terminal acidic region serves a regulatory role in controlling arrestin binding selectivity toward the phosphorylated and activated form of a receptor. The basic N-terminal domain directly participates in receptor interaction and serves a regulatory role via intramolecular interaction with the C-terminal acidic region. Also, two centrally localized domains are directly involved in determining receptor binding specificity and selectivity. (Ref.: Gurevich, VV et al. (1995). J. Biol. Chem. 270 (2); 720-731).

This rabbit polyclonal antibody detects Arrestin-C in mouse and rat retina. it targets an epitope with in 12 amino acids from the C-terminal end.

Synthetic Linear Peptide
Epitope: C-terminus

Research Sub Category
Sensory & PNS
Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected Cone Arrestin in rat retina, mouse retina, and mouse cerebral cortex tissue.

Research Category
Neuroscience
Anti-Cone Arrestin, Cat. No. AB15282, is a highly specific rabbit polyclonal antibody that targets Arrestin-C and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Evaluated by Western Blotting in mouse retinal tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected Cone Arrestin in 10 µg of mouse retinal tissue lysate.

ImmunoAffinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Concentration: Please refer to lot specific datasheet.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

rat, mouse

Chemicon^®

immunohistochemistry: suitable (paraffin)
western blot: suitable

IgG

NP_573468

Q9EQP6

wet ice

~41.9 kDa

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 2