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Viral RNA Extraction Buffer
| 产品编号： | 4080089 |
| 规格： | One Step RT-qPCR Compatible, Optimized for Viral RNA Detection in Saliva |
| 包装规格： | 0.5 ML，5 ML |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Viral RNA Extraction Buffer
Viral RNA Extraction
Viral Lysis Solution for RT-qPCR
For use with Reverse Transcription-qPCR
产品性质
| Quality Level【质量水平】 | 200 |
| grade【等级】 | Molecular Biology |
| form【形式】 | liquid |
| concentration【浓度】 | 4X |
| technique(s) | LAMP: suitable PCR: suitable RT-PCR: suitable qPCR: suitable |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | −20℃ |
基本信息
| General description【一般描述】 | The Viral RNA Extraction Buffer has been optimized to provide rapid, room-temperature lysis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral particles in saliva. The buffer comes as a 4x working solution that can be mixed with virus-containing samples in a qRT-PCR plate. Upon 5 minute incubation at room temperature, viral particles are lysed and RNA is stabilized such that samples are ready for direct analysis via RT-qPCR with no additional cleanup step. Removal of precipitates in saliva via centrifugation prior to testing is recommended for optimal sensitivity. |
| Application【应用】 | The Viral RNA Extraction Buffer is broadly compatible with most nucleic acid detection methods and does not reduce signal when used at working concentration in qRT-PCR. qRT-PCR compatibility has been demonstrated in a 4-fluor multiplex setting consisting of Cy5, TxRed, FAM, and HEX. RT-qPCR compatibility has been verified with several one-step RT-qPCR kits:
The Viral RNA Extraction Buffer is also compatible with colorimetric LAMP assays. |
| Features and Benefits【特点和优势】 |
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| Legal Information【法律信息】 | KiCqStart is a registered trademark of Qiagen Beverly Inc. ReadyMix is a trademark of sigma-aldrich Co. LLC |
安全信息
| Pictograms【象形图】 | GHS05 |
| Signal word【警示用语：】 | Danger |
| Hazard Statements | H318 |
| Precautionary Statements | P280 - P305 + P351 + P338 |
| Hazard Classifications【危险分类】 | Eye Dam. 1 |
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 3 |
